Effects of adenine nucleotide and sterol depletion on tight junction structure and function in MDCK cells
The antitumor agent Hadacidin (H), N-formyl-hydroxyamino-acetic acid, reversibly inhibited the multiplication of clone 4 Madin-Darby canine kidney (MDCK) cells at a 4 mM concentration within 24-48 hours. Treated cells were arrested in the S phase of the cell cycle. Accompanying this action was a 16-fold increase in the area occupied b the cells and a refractoriness to trypsin treatment. To test whether this effect was due to an increase in tight junction integrity, electrical resistance (TER) was measured across H-treated monolayers. Addition of H at the onset of junction formation reversibly prevented the development of TER. ATP and cAMP levels were decreased by H, as well as the rate of ({sup 3}H)-leucine incorporation into protein. When 1 mM dibutyryl-cAMP (d.cAMP) and theophylline were added, H had no effect on cell division or protein synthesis, and TER was partially restored. The addition of 1 mM d.cAMP and 1 mM theophylline to control cultures decreased TER, indicating a biphasic effect on TER development/maintenance. In a separate study, the effect of sterol depletion on tight junctions formation/maintenance in wild-type MDCK cells was investigated.
- Research Organization:
- Lowell Univ., MA (USA)
- OSTI ID:
- 6810425
- Resource Relation:
- Other Information: Thesis (Ph. D.)
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
ADENINES
BIOCHEMICAL REACTION KINETICS
ANIMAL CELLS
BIOLOGICAL FUNCTIONS
STEROLS
AMP
ATP
CELL CYCLE
DOGS
KIDNEYS
LEUCINE
MOLECULAR STRUCTURE
THEOPHYLLINE
TRACER TECHNIQUES
TRITIUM COMPOUNDS
TRYPSIN
AMINES
AMINO ACIDS
ANIMALS
ANTIMETABOLITES
AROMATICS
AZAARENES
BODY
CARBOXYLIC ACIDS
CARDIOVASCULAR AGENTS
DRUGS
ENZYMES
FUNCTIONS
HETEROCYCLIC COMPOUNDS
HYDROGEN COMPOUNDS
HYDROLASES
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
KINETICS
MAMMALS
NUCLEOTIDES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
ORGANS
PEPTIDE HYDROLASES
PURINES
REACTION KINETICS
SERINE PROTEINASES
STEROIDS
VASODILATORS
VERTEBRATES
XANTHINES
550201* - Biochemistry- Tracer Techniques