A quantitative analysis of 2-D gels identifies proteins in which labeling is increased following long-term sensitization in Aplysia

Journal Article · Wed Jun 01 00:00:00 EDT 1988 · Neuron (Cambridge, Massachusetts); (USA)

DOI:https://doi.org/10.1016/0896-6273(88)90080-3· OSTI ID:6810148

Castellucci, V F; Kennedy, T E; Kandel, E R; Goelet, P ^[1]

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1. Columbia Univ., New York, NY (USA)

Long-term memory for sensitization of the gill- and siphon-withdrawal reflex in Aplysia, produced by 4 days of training, is associated with increased synaptic efficacy of the connection between the sensory and motor neurons. This training is also accompanied by neuronal growth; there is an increase in the number of synaptic varicosities per sensory neuron and in the number of active zones. Such structural changes may be due to changes in the rates of synthesis of certain proteins. We have searched for proteins in which the rates of ({sup 35}S)methionine labeling are altered during the maintenance phase of long-term memory for sensitization by using computer-assisted quantitative 2-D gel analysis. This method has allowed us to detect 4 proteins in which labeling is altered after 4 days of sensitization training.

• OSTI ID: 6810148
• Journal Information: Neuron (Cambridge, Massachusetts); (USA), Vol. 1:4; ISSN 0896-6273
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
PROTEINS
QUANTITATIVE CHEMICAL ANALYSIS
AUTORADIOGRAPHY
LABELLING
METHIONINE
MOLECULAR STRUCTURE
NERVES
SULFUR 35
TWO-DIMENSIONAL ELECTROPHORESIS
AMINO ACIDS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CARBOXYLIC ACIDS
CHEMICAL ANALYSIS
DAYS LIVING RADIOISOTOPES
DRUGS
ELECTROPHORESIS
EVEN-ODD NUCLEI
ISOTOPES
LIGHT NUCLEI
LIPOTROPIC FACTORS
NERVOUS SYSTEM
NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
RADIOISOTOPES
SULFUR ISOTOPES
550201* - Biochemistry- Tracer Techniques