Effects of elevated temperature on protein breakdown in muscles from septic rats

Journal Article · Sun Apr 01 00:00:00 EST 1990 · American Journal of Physiology; (USA)

OSTI ID:6808813

Hall-Angeras, M A; Angeras, U H; Hasselgren, P O; Fischer, J E ^[1]

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1. Univ. of Cincinnati Medical Center, OH (USA)

Elevated temperature has been proposed to contribute to accelerated muscle protein degradation during fever and sepsis. The present study examined the effect of increased temperature in vitro on protein turnover in skeletal muscles from septic and control rats. Sepsis was induced by cecal ligation and puncture (CLP); control rats were sham operated. After 16 h, the extensor digitorum longus (EDL) and soleus (SOL) muscles were incubated at 37 or 40 degrees C. Protein synthesis was determined by measuring incorporation of (14C)phenylalanine into protein. Total and myofibrillar protein breakdown was assessed from release of tyrosine and 3-methylhistidine (3-MH), respectively. Total protein breakdown was increased at 40 degrees C by 15% in EDL and by 29% in SOL from control rats, whereas 3-MH release was not affected. In muscles from septic rats, total and myofibrillar protein breakdown was increased by 22 and 30%, respectively, at 40 degrees C in EDL but was not altered in SOL. Protein synthesis was unaffected by high temperature both in septic and nonseptic muscles. The present results suggest that high temperature is not the primary mechanism of increased muscle protein breakdown in sepsis because the typical response to sepsis, i.e., a predominant increase in myofibrillar protein breakdown, was not induced by elevated temperature in normal muscle. It is possible, however, that increased temperature may potentiate protein breakdown that is already stimulated by sepsis because elevated temperature increased both total and myofibrillar protein breakdown in EDL from septic rats.

• OSTI ID: 6808813
• Journal Information: American Journal of Physiology; (USA), Vol. 258; ISSN 0002-9513
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BACTERIAL DISEASES
PATHOGENESIS
HEAT
BIOLOGICAL EFFECTS
PROTEINS
METABOLISM
CARBON 14 COMPOUNDS
HISTIDINE
IN VITRO
ISOTOPE DILUTION
MUSCLES
PHENYLALANINE
RATS
TEMPERATURE EFFECTS
TYROSINE
AMINO ACIDS
ANIMALS
AZOLES
CARBOXYLIC ACIDS
DISEASES
ENERGY
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
HYDROXY ACIDS
IMIDAZOLES
INFECTIOUS DISEASES
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MAMMALS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
RODENTS
TRACER TECHNIQUES
VERTEBRATES
550901* - Pathology- Tracer Techniques
560200 - Thermal Effects