Plasmids containing the gene for DNA polymerase I from Streptococcus pneumoniae

Patent · Fri Aug 28 00:00:00 EDT 1987

OSTI ID:6805409

Lacks, S A; Martinez, S; Lopez, P; Espinosa, M

A method is disclosed for cloning the gene which encodes a DNA polymerase-exonuclease of /und Streptococcus/ /und pneumoniae/. Plasmid pSM22, the vector containing the pneumococcal polA gene, facilitates the expression of 50-fold greater amounts of the PolI enzyme. 1 fig., 1 tab.

• Research Organization: Brookhaven National Laboratory (BNL), Upton, NY (United States)
• DOE Contract Number: AC02-76CH00016
• Assignee: TIC; EDB-88-179643
• Patent Number(s): PATENTS-US-A6090623
• OSTI ID: 6805409
• Resource Relation: Other Information: Portions of this document are illegible in microfiche products
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
GENES
DNA-CLONING
BACILLUS SUBTILIS
DNA POLYMERASES
DNA REPLICATION
DNA SEQUENCING
INVENTIONS
PLASMIDS
STREPTOCOCCUS
TRACER TECHNIQUES
BACILLUS
BACTERIA
CELL CONSTITUENTS
CLONING
ENZYMES
ISOTOPE APPLICATIONS
MICROORGANISMS
NUCLEIC ACID REPLICATION
NUCLEOTIDYLTRANSFERASES
PHOSPHORUS-GROUP TRANSFERASES
POLYMERASES
STRUCTURAL CHEMICAL ANALYSIS
TRANSFERASES
550200* - Biochemistry