Mitochondrial DNA polymerase from embryos of Drosophila melanogaster: purification, subunit structure, and partial characterization
The mitochondrial DNA polymerase has been purified to near-homogeneity from early embryos of Drosophila melanogaster. Sodium dodecyl sulfate gel electrophoresis of the highly purified enzyme reveals two polypeptides with molecular masses of 125,000 and 35,000 daltons, in a ratio of 1:1. The enzyme has a sedimentation coefficient of 7.6 S and a stokes radius of 51 A. Taken together, the data suggest that the D. melanogaster DNA polymerase ..gamma.. is a heterodimer. DNA polymerase activity gel analysis has allowed the assignment of the DNA polymerization function to the large subunit. The DNA polymerase exhibits a remarkable ability to utilize efficiently a variety of template-primers including gapped DNA, poly(rA).oligo(dT) and singly primed phiX174 DNA. Both the crude and the highly purified enzymes are stimulated by KCl, and inhibited by dideoxythymidine triphosphate and by N-ethylmaleimide. Thus, the catalytic properties of the near-homogeneous Drosophila enzyme are consistent with those of DNA polymerase ..gamma.. as partially purified from several vertebrates.
- Research Organization:
- Michigan State Univ., East Lansing
- OSTI ID:
- 6802835
- Journal Information:
- J. Biol. Chem.; (United States), Vol. 261:31
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
DNA
BIOSYNTHESIS
DNA POLYMERASES
MOLECULAR STRUCTURE
PURIFICATION
DROSOPHILA
ENZYME ACTIVITY
LABELLED COMPOUNDS
ELECTROPHORESIS
EMBRYOS
MITOCHONDRIA
NUCLEOTIDES
PHOSPHORUS 32
TRITIUM COMPOUNDS
ANIMALS
ARTHROPODS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CELL CONSTITUENTS
DAYS LIVING RADIOISOTOPES
DIPTERA
ENZYMES
FLIES
FRUIT FLIES
INSECTS
INVERTEBRATES
ISOTOPES
LIGHT NUCLEI
NUCLEI
NUCLEIC ACIDS
NUCLEOTIDYLTRANSFERASES
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANOIDS
PHOSPHORUS ISOTOPES
PHOSPHORUS-GROUP TRANSFERASES
POLYMERASES
RADIOISOTOPES
SYNTHESIS
TRANSFERASES
550201* - Biochemistry- Tracer Techniques