Cross-linking of hCG to luteal receptors

Journal Article · Tue Jan 01 00:00:00 EST 1985 · Methods Enzymol.; (United States)

DOI:https://doi.org/10.1016/0076-6879(85)09086-3· OSTI ID:6801668

Ji, T H; Ji, I

Photoaffinity labeling of the lutropin/choriogonadotropin (LH/hCG) receptor system on porcine granulosa cells has demonstrated that both the ..cap alpha.. and ..beta.. subunits of hCG directly photoaffinity label the hormone receptor. Three new bands appear on SDS-PAGE as a consequence of photoaffinity labeling by each subunit: the molecular weights of the three bands (106K, 88K, and 83K) produced by the subunit are larger by approximately 10K than those of the three bands (96K, 76K, and 73K) labeled by the ..cap alpha.. subunit. Although it could be a coincidence that the molecular weight of the ..beta.. subunit is approximately 10K larger than that of the ..cap alpha.. subunit, the similarity in these differences suggests the possibility that both the ..cap alpha.. and ..beta.. subunits have labeled the same polypeptides.

• OSTI ID: 6801668
• Journal Information: Methods Enzymol.; (United States), Vol. 109
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
38 RADIATION CHEMISTRY, RADIOCHEMISTRY, AND NUCLEAR CHEMISTRY
HCG
RADIORECEPTOR ASSAY
LH
RECEPTORS
LABELLING
ANIMAL CELLS
BIOCHEMICAL REACTION KINETICS
EXPERIMENTAL DATA
IODINE 125
LABELLED COMPOUNDS
MOLECULAR WEIGHT
PHOTOLYSIS
SWINE
TRACER TECHNIQUES
ANIMALS
BETA DECAY RADIOISOTOPES
CHEMICAL REACTIONS
DATA
DAYS LIVING RADIOISOTOPES
DECOMPOSITION
DOMESTIC ANIMALS
ELECTRON CAPTURE RADIOISOTOPES
GONADOTROPINS
HORMONES
INFORMATION
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
MAMMALS
MEMBRANE PROTEINS
NUCLEI
NUMERICAL DATA
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PEPTIDE HORMONES
PHOTOCHEMICAL REACTIONS
PITUITARY HORMONES
PROTEINS
RADIOISOTOPES
REACTION KINETICS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques
400700 - Radiochemistry & Nuclear Chemistry