Cytogenetic comparison of the responses of mouse and human peripheral blood lymphocytes to /sup 60/Co gamma radiation
Experiments were conducted to compare the chromosome damaging effects of /sup 60/Co gamma radiation on mouse and human peripheral blood lymphocytes (PBLs). Either whole blood or isolated and pelleted mononuclear leucocytes (MNLs) were irradiated with a /sup 60/Co unit to yield exposures of 1, 2, 3, or 4 Gy. In addition, mice were whole-body irradiated in vivo with the same doses so that an in vitro-in vivo comparison could be made. The results indicate that mouse PBLs irradiated in whole blood, whether in vivo or in vitro, respond similarly to /sup 60/Co gamma rays as measured by dicentric chromosome formation. In addition, mouse and human PBLs showed a similar radiosensitivity, but because the mouse PBL data were best fitted to an exponential function and the human PBL data to a quadratic function, direct comparisons were difficult to make. Pelleted MNLs from mice were much less sensitive to the clastogenic effects of gamma radiation than whole blood. This is believed to be due to hypoxic conditions that developed during irradiation and transport. Human PBLs did not show a marked difference whether irradiated in whole blood or as pelleted MNLs in tissue culture medium.
- Research Organization:
- Environmental Protection Agency, Research Triangle Park, NC (USA)
- OSTI ID:
- 6801154
- Journal Information:
- Radiat. Res.; (United States), Vol. 115:2
- Country of Publication:
- United States
- Language:
- English
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LYMPHOCYTES
GENETIC RADIATION EFFECTS
BLOOD
CHROMOSOMES
COBALT 60
COMPARATIVE EVALUATIONS
DOSE-RESPONSE RELATIONSHIPS
GAMMA RADIATION
MAN
MICE
RADIOSENSITIVITY
WHOLE-BODY IRRADIATION
ANIMAL CELLS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BIOLOGICAL RADIATION EFFECTS
BLOOD CELLS
BODY FLUIDS
COBALT ISOTOPES
CONNECTIVE TISSUE CELLS
ELECTROMAGNETIC RADIATION
EXTERNAL IRRADIATION
GENETIC EFFECTS
INTERMEDIATE MASS NUCLEI
INTERNAL CONVERSION RADIOISOTOPES
IONIZING RADIATIONS
IRRADIATION
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
LEUKOCYTES
MAMMALS
MATERIALS
MINUTES LIVING RADIOISOTOPES
NUCLEI
ODD-ODD NUCLEI
PRIMATES
RADIATION EFFECTS
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RODENTS
SOMATIC CELLS
VERTEBRATES
YEARS LIVING RADIOISOTOPES
560120* - Radiation Effects on Biochemicals
Cells
& Tissue Culture
560152 - Radiation Effects on Animals- Animals