Unique pattern of interleukin 2 receptor expression by lymphocytes in response to anti-LEU 4: relationship to monocyte accessory cell function
The relationship between early interleukin 2 receptor (IL2R) expression and subsequent DNA synthesis by mitogen-stimulated human mononuclear cells (MC) was studied. For serial dilutions of a given mitogen, the percentage of lymphocytes expressing IL2R after 1 day of culture was plotted vs /sup 3/H-thymidine incorporation on day 3, and the IL2R value associated with 50,000 counts per minute (IL2R-50K) determined. A mean IL2R-50K value of 7 characterized PHA, Con A, and OKT3 responses, while a higher value of 29 characterized anti-Leu 4 (L4) responses. As tested on OKT3 and L4 systems, exogenous IL2 did not alter IL2R-50K values. Because OKT3 and L4 both recognize the lymphocyte CD3 antigen, but react with different monocyte Fc receptors, the role of monocytes in producing elevated L4 IL2R-50K values was explored. MC from healthy L4 nonresponders (NR), induced to proliferate by L4 in the presence of responder (R) monocytes, also yielded an elevated mean IL2R-50K value of 31. In contrast, direct stimulation of R-MC, NR-MC, or NR-MC plus R monocytes by L4-coated sepharose beads produced mean IL2R-50K values of 12 or 13. These findings suggest that crosslinking of lymphocyte-bound soluble L4 by R monocytes leads to uniquely elevated pattern of lymphocyte IL2R expression. Crosslinking mediated by L4-beads, however, leads to a more conventional pattern of IL2R expression, even though R monocytes may be present.
- Research Organization:
- Americal Red Cross Blood Services, Los Angeles, CA
- OSTI ID:
- 6798388
- Report Number(s):
- CONF-8604222-
- Journal Information:
- Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 45:4; Conference: 70. annual meeting of the Federation of American Society for Experimental Biology, St. Louis, MO, USA, 13 Apr 1986
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
LYMPHOCYTES
CELL PROLIFERATION
LYMPHOKINES
RECEPTORS
MONOCYTES
BIOLOGICAL FUNCTIONS
ANTIGENS
CROSS-LINKING
DNA REPLICATION
MITOGENS
THYMIDINE
TRACER TECHNIQUES
TRITIUM COMPOUNDS
ANIMAL CELLS
AZINES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CHEMICAL REACTIONS
CONNECTIVE TISSUE CELLS
FUNCTIONS
GROWTH FACTORS
HETEROCYCLIC COMPOUNDS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LEUKOCYTES
MATERIALS
MEMBRANE PROTEINS
NUCLEIC ACID REPLICATION
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
POLYMERIZATION
PROTEINS
PYRIMIDINES
RIBOSIDES
SOMATIC CELLS
550201* - Biochemistry- Tracer Techniques