New method to quantitate platelets adhered on biomaterials using monoclonal antibodies to human platelet membrane glycoprotein SZ-21

Journal Article · Mon Jan 01 00:00:00 EST 1990 · Biomaterials Artificial Cells and Artificial Organs; (USA)

OSTI ID:6795359

Xi, T F; Zhang, J C; Tian, W H; Wang, C R; Lei, X H; Wai, H Y; Ruan, C G ^[1]

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1. National Institute for Control of Pharmaceutical and Biological Products, Beijing (China)

This study developed a new technique to quantitate platelets adhered on biomaterials surfaces in vitro, based on a surface phased radioimmunoassay using a monoclonal antibody SZ-21, directed specifically against the membrane glycoprotein complex IIIa of human platelets. In vitro perfusion is performed in system which consists of testing tubes and infusion pump. After 5 minutes perfusion with fresh ACD anticoagulated human whole blood at 2,000s-1 platelets deposition on surface precoated with proteins determined using anti-human platelet antibody (125 I-SZ-21) are 4,173 +/- 932 (Albumin), 59,032 +/- 25,554 (Fibrinogen), and 71,253 +/- 11,484 (Collagen). Meanwhile, platelets adhered on surfaces of four polymers were determined (platelet/mm2): 19,493 +/- 2,050 (Silicone), 48,193 +/- 4,055 (Polytetrafluoroethylene), 50,375 +/- 8,675 (Polyvinyl chloride) and 101,906 +/- 5,916 (Polyethylene). These results were confirmed by SEM. This method is not only applied for evaluating rapidly and reliably blood compatibility of biomaterials in vitro, but will be used at basic study for interaction of blood materials.

• OSTI ID: 6795359
• Journal Information: Biomaterials Artificial Cells and Artificial Organs; (USA), Vol. 18:3; ISSN 0890-5533
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
BLOOD PLATELETS
ADHESION
MONOCLONAL ANTIBODIES
SPECIFICITY
ALBUMINS
COLLAGEN
FIBRINOGEN
GLYCOPROTEINS
IN VITRO
MAN
PERFUSED TISSUES
RADIOIMMUNOASSAY
ANIMAL TISSUES
ANIMALS
ANTIBODIES
BIOASSAY
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BLOOD COAGULATION FACTORS
BODY
BODY FLUIDS
COAGULANTS
DIAGNOSTIC TECHNIQUES
DRUGS
GLOBULINS
HEMATOLOGIC AGENTS
HEMOSTATICS
IMMUNOASSAY
IMMUNOLOGY
ISOTOPE APPLICATIONS
MAMMALS
MATERIALS
ORGANIC COMPOUNDS
PRIMATES
PROTEINS
RADIOASSAY
RADIOIMMUNODETECTION
RADIOIMMUNOLOGY
SCLEROPROTEINS
TISSUES
TRACER TECHNIQUES
VERTEBRATES
550201* - Biochemistry- Tracer Techniques