Interference by human anti-mouse antibodies in CA 125 assay after immunoscintigraphy: Anti-idiotypic antibodies not neutralized by mouse IgG but removed by chromatography

Journal Article · Sun Jul 01 00:00:00 EDT 1990 · Clinical Chemistry (Winston-Salem, North Carolina); (USA)

OSTI ID:6793058

Turpeinen, U; Lehtovirta, P; Alfthan, H; Stenman, U H ^[1]

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1. Helsinki Univ. Central Hospital (Finland)

Falsely increased concentrations of the ovarian carcinoma-associated antigen, CA 125, were measured by a monoclonal antibody (MAb)-based double determinant immunoradiometric assay (IRMA) in patients who developed antibodies to mouse immunoglobulins (IgGs) after receiving injections of the same MAb as is used in the CA 125 IRMA. Addition of undiluted mouse serum or purified mouse IgG to the assay mixture failed to eliminate the falsely increased CA 125 concentrations in most of the samples, owing to the presence of anti-idiotype antibody. Because of their anti-idiotypic nature, the human anti-mouse antibodies (HAMAS) had only little effect on other immunometric assays, and this effect could be completely eliminated by addition of mouse IgG. To eliminate the effect of HAMA on the CA 125 assay, we studied the ability of various chromatographic methods to separate the interfering HAMA from CA 125. For measuring HAMA in serum and chromatographic fractions we developed a time-resolved fluoroimmunoassay. Adequate separation of CA 125 and HAMA was achieved by affinity chromatography of patients' sera with solid-phase Protein A, Protein G, cation-exchange chromatography on Mono S, and gel filtration on Superose 6. These results demonstrate that the interference can effectively be removed by rather simple chromatographic procedures.

• OSTI ID: 6793058
• Journal Information: Clinical Chemistry (Winston-Salem, North Carolina); (USA), Vol. 36:7; ISSN 0009-9147
• Country of Publication: United States
• Language: English

Related Subjects

62 RADIOLOGY AND NUCLEAR MEDICINE
CARCINOMAS
DIAGNOSIS
MONOCLONAL ANTIBODIES
SPECIFICITY
OVARIES
IMMUNOGLOBULINS
INTERFERENCE
ION EXCHANGE CHROMATOGRAPHY
MICE
PATIENTS
RADIOIMMUNOASSAY
RADIOIMMUNOSCINTIGRAPHY
ANIMALS
ANTIBODIES
BIOASSAY
BODY
CHROMATOGRAPHY
COUNTING TECHNIQUES
DIAGNOSTIC TECHNIQUES
DISEASES
FEMALE GENITALS
GLOBULINS
GONADS
IMMUNOASSAY
IMMUNOLOGY
ISOTOPE APPLICATIONS
MAMMALS
NEOPLASMS
ORGANIC COMPOUNDS
ORGANS
PROTEINS
RADIOASSAY
RADIOIMMUNODETECTION
RADIOIMMUNOLOGY
RADIOISOTOPE SCANNING
RODENTS
SCINTISCANNING
SEPARATION PROCESSES
TRACER TECHNIQUES
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550601* - Medicine- Unsealed Radionuclides in Diagnostics