The SRS2 suppressor of rad6 mutations of Saccharomyces cerevisiae acts by channeling DNA lesions into the RAD52 DNA repair pathway

Journal Article · Sun Apr 01 00:00:00 EST 1990 · Genetics; (USA)

OSTI ID:6790649

Schiestl, R H; Prakash, S; Prakash, L ^[1]

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1. Univ. of Rochester School of Medicine, NY (USA)

rad6 mutants of Saccharomyces cerevisiae are defective in the repair of damaged DNA, DNA damage induced mutagenesis, and sporulation. In order to identify genes that can substitute for RAD6 function, the authors have isolated genomic suppressors of the UV sensitivity of rad6 deletion (rad6{Delta}) mutations and show that they also suppress the {gamma}-ray sensitivity but not the UV mutagenesis or sporulation defects of rad6. The suppressors show semidominance for suppression of UV sensitivity and dominance for suppression of {gamma}-ray sensitivity. The six suppressor mutations they isolated are all alleles of the same locus and are also allelic to a previously described suppressor of the rad6-1 nonsense mutation, SRS2. They show that suppression of rad6{Delta} is dependent on the RAD52 recombinational repair pathway since suppression is not observed in the rad6{Delta} SRS2 strain containing an additional mutation in either the RAD51, RAD52, RAD54, RAD55 or RAD57 genes. Possible mechanisms by which SRS2 may channel unrepaired DNA lesions into the RAD52 DNA repair pathway are discussed.

• OSTI ID: 6790649
• Journal Information: Genetics; (USA), Vol. 124:4; ISSN 0016-6731
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
DNA REPAIR
GENE MUTATIONS
SACCHAROMYCES CEREVISIAE
GENETIC RADIATION EFFECTS
BIOLOGICAL PATHWAYS
COBALT 60
DNA
GAMMA RADIATION
MUTAGENESIS
MUTATION FREQUENCY
ULTRAVIOLET RADIATION
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
COBALT ISOTOPES
ELECTROMAGNETIC RADIATION
EUMYCOTA
FUNGI
GENETIC EFFECTS
INTERMEDIATE MASS NUCLEI
INTERNAL CONVERSION RADIOISOTOPES
IONIZING RADIATIONS
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
MICROORGANISMS
MINUTES LIVING RADIOISOTOPES
MUTATIONS
NUCLEI
NUCLEIC ACIDS
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PLANTS
RADIATION EFFECTS
RADIATIONS
RADIOISOTOPES
RECOVERY
REPAIR
SACCHAROMYCES
YEARS LIVING RADIOISOT
YEASTS
560120* - Radiation Effects on Biochemicals
Cells
& Tissue Culture