Polydiacetylene liposomes functionalized with sialic acid bind and colorimetrically detect influenza virus

Journal Article · Wed Jan 18 00:00:00 EST 1995 · Journal of the American Chemical Society; (United States)

DOI:https://doi.org/10.1021/ja00107a032· OSTI ID:6790533

Reichert, A; Nagy, J O; Spevak, W; Charych, D ^[1]

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1. Lawrence Berkeley Lab., CA (United States)

In this paper we have demonstrated that polymerized liposomes are biomolecular materials that provide a molecular recognition function (sialic acid) and a detection element (polydiacetylene backbone), all within a single supramolecular assembly. The binding event is transduced to a visible color change, readily seen with the naked eye and quantified by absorption spectroscopy. Specificity of the color change was demonstrated by competitive inhibition studies. In addition, nonspecific adsorption, if it occurs. does not appear to affect the color of the liposome solutions. 28 refs., 2 figs.

• DOE Contract Number: AC03-76SF00098
• OSTI ID: 6790533
• Journal Information: Journal of the American Chemical Society; (United States), Vol. 117:2; ISSN 0002-7863
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
37 INORGANIC
ORGANIC
PHYSICAL AND ANALYTICAL CHEMISTRY
INFLUENZA VIRUSES
DETECTION
OPTICAL PROPERTIES
LIPOSOMES
ABSORPTION SPECTROSCOPY
CHEMICAL PREPARATION
ALKYNES
SIALIC ACID
CARBOHYDRATES
HYDROCARBONS
MICROORGANISMS
MONOSACCHARIDES
ORGANIC COMPOUNDS
PARASITES
PHYSICAL PROPERTIES
SACCHARIDES
SPECTROSCOPY
SYNTHESIS
VIRUSES
550700* - Microbiology
550200 - Biochemistry
400201 - Chemical & Physicochemical Properties
400102 - Chemical & Spectral Procedures