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Title: Isolation and characterization of mink lung epithelial cell mutants resistant to transforming growth factor. beta

Abstract

Mink lung epithelial cells resistant to growth inhibition by transforming growth factor ..beta.. (TGF-..beta..) have been isolated by chemical mutagenesis and growth in the presence of platelet extracts enriched in TGF-..beta... Several resistant clones were isolated, at least one of which stably retained its resistance to TGF-..beta.. when grown in the absence of the factor. The cells of this clone were similar to the parent cells in morphology and growth properties. However, unlike the parent cells, the resistant cells did not show any of the following responses to /sup 125/I TGF-..beta..: (1) inhibition of DNA synthesis and proliferation; (2) morphological changes involving increased cell spreading; or (3) stimulation of synthesis of a 48-kilodalton secreted /sup 35/S-protein. The resistant cells do, however, retain a functional TGF-..beta.. receptor. The TGF-..beta.. resistant cell lines may be useful in genetic studies designed to identify the biochemical events required for inhibition of epithelial cell growth by this factor.

Authors:
Publication Date:
Research Org.:
Vanderbilt Univ. School of Medicine, Nashville, TN
OSTI Identifier:
6790470
Resource Type:
Journal Article
Journal Name:
J. Cell. Physiol.; (United States)
Additional Journal Information:
Journal Volume: 130:1
Country of Publication:
United States
Language:
English
Subject:
62 RADIOLOGY AND NUCLEAR MEDICINE; 59 BASIC BIOLOGICAL SCIENCES; EPITHELIUM; MORPHOLOGICAL CHANGES; MUTANTS; BIOCHEMISTRY; PROTEINS; RADIORECEPTOR ASSAY; BLOOD PLATELETS; CELL PROLIFERATION; DNA REPLICATION; GROWTH; IODINE 125; LUNGS; METHIONINE; SULFUR 35; THYMIDINE; TRITIUM COMPOUNDS; AMINO ACIDS; ANIMAL TISSUES; AZINES; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; BIOLOGICAL MATERIALS; BLOOD; BLOOD CELLS; BODY; BODY FLUIDS; CARBOXYLIC ACIDS; CHEMISTRY; DAYS LIVING RADIOISOTOPES; DRUGS; ELECTRON CAPTURE RADIOISOTOPES; EVEN-ODD NUCLEI; HETEROCYCLIC COMPOUNDS; INTERMEDIATE MASS NUCLEI; IODINE ISOTOPES; ISOTOPE APPLICATIONS; ISOTOPES; LABELLED COMPOUNDS; LIGHT NUCLEI; LIPOTROPIC FACTORS; MATERIALS; NUCLEI; NUCLEIC ACID REPLICATION; NUCLEOSIDES; NUCLEOTIDES; ODD-EVEN NUCLEI; ORGANIC ACIDS; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; ORGANIC SULFUR COMPOUNDS; ORGANS; PYRIMIDINES; RADIOISOTOPES; RESPIRATORY SYSTEM; RIBOSIDES; SULFUR ISOTOPES; TISSUES; TRACER TECHNIQUES; 550601* - Medicine- Unsealed Radionuclides in Diagnostics; 550301 - Cytology- Tracer Techniques

Citation Formats

Chinkers, M. Isolation and characterization of mink lung epithelial cell mutants resistant to transforming growth factor. beta. United States: N. p., 1987. Web. doi:10.1002/jcp.1041300102.
Chinkers, M. Isolation and characterization of mink lung epithelial cell mutants resistant to transforming growth factor. beta. United States. https://doi.org/10.1002/jcp.1041300102
Chinkers, M. 1987. "Isolation and characterization of mink lung epithelial cell mutants resistant to transforming growth factor. beta". United States. https://doi.org/10.1002/jcp.1041300102.
@article{osti_6790470,
title = {Isolation and characterization of mink lung epithelial cell mutants resistant to transforming growth factor. beta},
author = {Chinkers, M},
abstractNote = {Mink lung epithelial cells resistant to growth inhibition by transforming growth factor ..beta.. (TGF-..beta..) have been isolated by chemical mutagenesis and growth in the presence of platelet extracts enriched in TGF-..beta... Several resistant clones were isolated, at least one of which stably retained its resistance to TGF-..beta.. when grown in the absence of the factor. The cells of this clone were similar to the parent cells in morphology and growth properties. However, unlike the parent cells, the resistant cells did not show any of the following responses to /sup 125/I TGF-..beta..: (1) inhibition of DNA synthesis and proliferation; (2) morphological changes involving increased cell spreading; or (3) stimulation of synthesis of a 48-kilodalton secreted /sup 35/S-protein. The resistant cells do, however, retain a functional TGF-..beta.. receptor. The TGF-..beta.. resistant cell lines may be useful in genetic studies designed to identify the biochemical events required for inhibition of epithelial cell growth by this factor.},
doi = {10.1002/jcp.1041300102},
url = {https://www.osti.gov/biblio/6790470}, journal = {J. Cell. Physiol.; (United States)},
number = ,
volume = 130:1,
place = {United States},
year = {Thu Jan 01 00:00:00 EST 1987},
month = {Thu Jan 01 00:00:00 EST 1987}
}