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Title: Radiation enhances silica translocation to the pulmonary interstitium and increases fibrosis in mice

Abstract

The effects of whole body irradiation (WBR) on particle clearance and the development of pulmonary fibrosis have been investigated. Using carbon, clearance is accomplished by polymorphonuclear leukocytes (PMN) and alveolar macrophages (AM), and only a few particles reach the interstitum. However, in preirradiated mice, the usual eflux of inflammatory cells is much delayed so that more free carbon remains in the alveoli, and by 1 week, many particles cross the epithelium to be phagocytized by interstitial macrophages. Carbon is found in the peribronchiolar interstitium 6 months later with no evidence of fibrosis. In the present study, mice received 1 mg silica intratracheally 2 days after 6.5 Gy WBR when the white blood cell count was low. A much-reduced Am and PMN response was found in the following 2 weeks compared to the reaction to silica alone, and many silica particles reached interstitial macrophages. In this case, macrophage activation by silica was associated with fibroblast proliferation, and by 16 weeks, much more pulmonary fibrosis was produced than after silica or irradiation only. This was measured biochemically and correlated with a large increase in retained silica in the irradiation-silica group. The results indicate that radiation inhibits the inflammatory response to particle instillation,more » resulting in greater translocation of free particles to the pulmonary interstitium. In the case of silica, the greater, prolonged interaction with interstitial macrophages leads to a much exaggerated fibrotic reaction. 17 refs., 11 figs.« less

Authors:
 [1]
  1. Univ. of Manitoba, Winnipeg (Canada)
Publication Date:
OSTI Identifier:
6790241
Resource Type:
Journal Article
Journal Name:
Environmental Health Perspectives; (United States)
Additional Journal Information:
Journal Volume: 97; Journal ID: ISSN 0091-6765
Country of Publication:
United States
Language:
English
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; 59 BASIC BIOLOGICAL SCIENCES; LUNGS; BIOLOGICAL RADIATION EFFECTS; FIBROSIS; MACROPHAGES; METABOLIC ACTIVATION; RADIOSENSITIVITY; SILICA; LUNG CLEARANCE; TRANSLOCATION; CARBON; LEUKOCYTES; MICE; WHOLE-BODY IRRADIATION; ANIMAL CELLS; ANIMALS; BIOLOGICAL EFFECTS; BIOLOGICAL MATERIALS; BLOOD; BLOOD CELLS; BODY; BODY FLUIDS; CHALCOGENIDES; CLEARANCE; CONNECTIVE TISSUE CELLS; ELEMENTS; EXCRETION; EXTERNAL IRRADIATION; IRRADIATION; MAMMALS; MATERIALS; MINERALS; NONMETALS; ORGANS; OXIDE MINERALS; OXIDES; OXYGEN COMPOUNDS; PATHOLOGICAL CHANGES; PHAGOCYTES; RADIATION EFFECTS; RESPIRATORY SYSTEM; RODENTS; SILICON COMPOUNDS; SILICON OXIDES; SOMATIC CELLS; VERTEBRATES; 560150* - Radiation Effects on Animals; 550500 - Metabolism; 550300 - Cytology; 550200 - Biochemistry

Citation Formats

Adamson, I Y.R. Radiation enhances silica translocation to the pulmonary interstitium and increases fibrosis in mice. United States: N. p., 1992. Web. doi:10.1289/ehp.9297233.
Adamson, I Y.R. Radiation enhances silica translocation to the pulmonary interstitium and increases fibrosis in mice. United States. https://doi.org/10.1289/ehp.9297233
Adamson, I Y.R. 1992. "Radiation enhances silica translocation to the pulmonary interstitium and increases fibrosis in mice". United States. https://doi.org/10.1289/ehp.9297233.
@article{osti_6790241,
title = {Radiation enhances silica translocation to the pulmonary interstitium and increases fibrosis in mice},
author = {Adamson, I Y.R.},
abstractNote = {The effects of whole body irradiation (WBR) on particle clearance and the development of pulmonary fibrosis have been investigated. Using carbon, clearance is accomplished by polymorphonuclear leukocytes (PMN) and alveolar macrophages (AM), and only a few particles reach the interstitum. However, in preirradiated mice, the usual eflux of inflammatory cells is much delayed so that more free carbon remains in the alveoli, and by 1 week, many particles cross the epithelium to be phagocytized by interstitial macrophages. Carbon is found in the peribronchiolar interstitium 6 months later with no evidence of fibrosis. In the present study, mice received 1 mg silica intratracheally 2 days after 6.5 Gy WBR when the white blood cell count was low. A much-reduced Am and PMN response was found in the following 2 weeks compared to the reaction to silica alone, and many silica particles reached interstitial macrophages. In this case, macrophage activation by silica was associated with fibroblast proliferation, and by 16 weeks, much more pulmonary fibrosis was produced than after silica or irradiation only. This was measured biochemically and correlated with a large increase in retained silica in the irradiation-silica group. The results indicate that radiation inhibits the inflammatory response to particle instillation, resulting in greater translocation of free particles to the pulmonary interstitium. In the case of silica, the greater, prolonged interaction with interstitial macrophages leads to a much exaggerated fibrotic reaction. 17 refs., 11 figs.},
doi = {10.1289/ehp.9297233},
url = {https://www.osti.gov/biblio/6790241}, journal = {Environmental Health Perspectives; (United States)},
issn = {0091-6765},
number = ,
volume = 97,
place = {United States},
year = {Wed Jul 01 00:00:00 EDT 1992},
month = {Wed Jul 01 00:00:00 EDT 1992}
}