Molecular characterization of the gene for human interleukin-1[beta] converting enzyme (IL1BC)
- Immunex Corp., Seattle, WA (United States)
- St. Jude Children's Research Hospital, Memphis, TN (United States)
- St. Jude Children's Research Hospital, Memphis, TN (United States) Univ. of Tennessee College of Medicine, Memphis, TN (United States)
Interleukin-1[beta] (IL-1[beta]) mediates a wide range of immune and inflammatory responses. The active cytokine is generated by proteolytic cleavage of an inactive precursor by a protease called the IL-1[beta] converting enzyme (ICE). A cDNA encoding this protease was recently isolated. A human genomic clone containing the ICE gene (IL1BC) was isolated using the cDNA as a probe. The gene consists of 10 exons spanning at least 10.6 kb. 5[prime]-anchored polymerase chain reaction indicated a single transcription start site [approximately]33 bp upstream of the initiator Met codon. The 5[prime]-flanking region does not have an apparent TATA box but may contain an initiator (Inr) promotor element. However, transcriptional activity could not be detected with a fusion gene containing the 5[prime]-flanking region linked to the bacterial chloramphenicol acetyltransferase gene (CAT) when transfected into the human acute monocytic leukemia cell line THP-1. Using the genomic IL1BC clone, the authors have confirmed the localization of the gene to chromosome 11 band q22.2-q22.3 by fluorescence in situ hybridization. 34 refs., 2 figs., 1 tab.
- OSTI ID:
- 6789210
- Journal Information:
- Genomics; (United States), Vol. 20:3; ISSN 0888-7543
- Country of Publication:
- United States
- Language:
- English
Similar Records
Genomic structure and chromosomal mapping of the human CD22 gene
The murine biglycan: Complete cDNA cloning, genomic organization, promoter function, and expression