Dose-related effects of psoralen and ultraviolet light on the cell cycle of murine melanoma cells

Journal Article · Tue Jun 01 00:00:00 EDT 1982 · Cancer Res.; (United States)

OSTI ID:6782740

Varga, J M; Wiesehahn, G; Bartholomew, J C; Hearst, J E

Cloudman (S91) murine melanoma cells were treated with 4'-hydroxymethyltrioxsalen (HMT), a bifunctional psoralen and exposed to long-wavelength (365 nm) ultraviolet light. DNA content of the cells stained with propidium iodide was measured by flow cytometry, and cell cycle phases were delineated from the DNA histograms by using a curve-fitting routine. Researchers found that HMT in combination with long-wavelength (365 nm) ultraviolet irradiation blocked melanoma cells in different phases of the cell cycle, depending on the dose of long-wavelength (365 nm) ultraviolet light and the concentration of HMT. The binding of (/sup 3/H)HMT to DNA was measured parallel with cell cycle analyses. Treatments with HMT at concentrations corresponding to about 1 HMT bound per 10(6) base pairs of DNA led to the accumulation of cells with predominantly G2 DNA content. At higher concentrations (2 to 3 HMT/10(6) base pairs), the cells were blocked in the S and G1 phases. In conclusion, it was shown that extremely sparse substitution of HMT to DNA blocks melanoma cells in the G2 phase or other phases of the cell cycle in a dose-dependent manner.

• Research Organization: Department of Dermatology, Yale University School of Medicine, New Haven, Connecticut
• OSTI ID: 6782740
• Journal Information: Cancer Res.; (United States), Vol. 42:6
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
59 BASIC BIOLOGICAL SCIENCES
CELL CULTURES
SENSITIVITY
MELANOMAS
BIOLOGICAL RADIATION EFFECTS
PSORALEN
RADIOSENSITIVITY EFFECTS
BINDING ENERGY
CELL CYCLE
DNA
DOSE-RESPONSE RELATIONSHIPS
MEASURING METHODS
MICE
QUANTITY RATIO
TRACER TECHNIQUES
TRITIUM COMPOUNDS
TUMOR CELLS
ULTRAVIOLET RADIATION
ANIMAL CELLS
ANIMALS
ANTICOAGULANTS
BIOLOGICAL EFFECTS
COUMARINS
DISEASES
DRUGS
ELECTROMAGNETIC RADIATION
ENERGY
HEMATOLOGIC AGENTS
HETEROCYCLIC COMPOUNDS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MAMMALS
NEOPLASMS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
RADIATION EFFECTS
RADIATIONS
RODENTS
VERTEBRATES
560121* - Radiation Effects on Cells- External Source- (-1987)
550301 - Cytology- Tracer Techniques