Determination of the intracellular pH of intact erythrocytes by /sup 1/H NMR spectroscopy

Journal Article · Thu Apr 01 00:00:00 EST 1982 · Anal. Biochem.; (United States)

DOI:https://doi.org/10.1016/0003-2697(82)90502-4· OSTI ID:6781394

Rabenstein, D L; Isab, A A

A method is described for determining the intracellular pH of intact erythrocytes by /sup 1/H NMR. The determination is based on the pH dependence of the chemical shifts of resonances for carbon-bounded protons of an indicator molecule (imidazole) in intact cells. The imidazole is introduced into the erythrocytes by incubation in an isotonic saline solution of the indicator. The pH dependence of the chemical shifts of the imidazole resonances is calibrated from /sup 1/H NMR spectra of the imidazole-containing red cell lysates whose pH is varied by the addition of acid or base and measured directly with a pH electrode. To reduce in intensity or eliminate the much more intense envelope of resonances from the hemoglobin, the /sup 1/H NMR measurements are made by either the spin-echo Fourier transform technique or by the transfer-or-saturation by cross-relaxation method.

• Research Organization: Univ. of Alberta, Edmonton
• OSTI ID: 6781394
• Journal Information: Anal. Biochem.; (United States), Vol. 121:2
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
ERYTHROCYTES
NMR SPECTRA
PH VALUE
HYDROGEN 1
NUCLEAR MAGNETIC RESONANCE
CHEMICAL SHIFT
IMIDAZOLES
RESONANCE
SPIN ECHO
AZOLES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
HETEROCYCLIC COMPOUNDS
HYDROGEN ISOTOPES
ISOTOPES
LIGHT NUCLEI
MAGNETIC RESONANCE
MATERIALS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
SPECTRA
STABLE ISOTOPES
550201* - Biochemistry- Tracer Techniques