Radioimmunoassay for quantitation of 2,3,7,8-tetrachlorodibenzofuran
A radioimmunoassay is described for the determination of 2,3,7,8-tetrachlorodibenzofuran (TCDF) in commercial preparations of polychlorinated biphenyls (PCBs) as well as environmental samples including animal tissues. A feature of the assay method is the use of nonionic detergent to solubilize the hydrophobic dibenzofurans in a manner permitting their binding to antibodies. The detection limits of the assay range between 20 pg and 4.0 ng of 2,3,7,8-TCDF. Extensive cross-reactivity studies indicated that the antiserum was fairly specific for TCDF although some cross-reactivity was found to structurally similar compounds. Values for TCDF in various Aroclors and animal tissues by radioimmunoassay correlated with those obtained by gas chromatography-mass spectrometry analysis. The radioimmunoassay is applicable to screening samples in order to minimize the demand for mass spectrometric screening and should be simple enough to be performed in most clinical laboratories. 3 tables, 1 figure.
- Research Organization:
- National Inst. of Environmental Health Sciences, Research Triangle Park, NC
- OSTI ID:
- 6776790
- Journal Information:
- Anal. Chem.; (United States), Vol. 52:9
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
ORGANIC
PHYSICAL AND ANALYTICAL CHEMISTRY
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
CHLORINATED AROMATIC HYDROCARBONS
RADIOIMMUNOASSAY
ANTIBODIES
BIPHENYL
ENVIRONMENTAL MATERIALS
FURANS
GAS CHROMATOGRAPHY
MASS SPECTROSCOPY
ORGANIC COMPOUNDS
QUANTITATIVE CHEMICAL ANALYSIS
TISSUES
AROMATICS
BODY
CHEMICAL ANALYSIS
CHROMATOGRAPHY
HALOGENATED AROMATIC HYDROCARBONS
HETEROCYCLIC COMPOUNDS
HYDROCARBONS
ISOTOPE APPLICATIONS
MATERIALS
ORGANIC CHLORINE COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
RADIOASSAY
SEPARATION PROCESSES
SPECTROSCOPY
TRACER TECHNIQUES
400103* - Radiometric & Radiochemical Procedures- (-1987)
560305 - Chemicals Metabolism & Toxicology- Vertebrates- (-1987)