Effect of pressure on an enzymatic reaction in a supercritical fluid

Journal Article · Thu Feb 01 00:00:00 EST 1990 · A.I.Ch.E. Journal (American Institute of Chemical Engineers); (USA)

DOI:https://doi.org/10.1002/aic.690360218· OSTI ID:6775097

Erickson, J C; Schyns, P; Cooney, C L ^[1]

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1. Massachusetts Inst. of Tech., Cambridge, MA (USA). Dept. of Chemical Engineering

Three different authors have reported on the use of four different enzymes in supercritical fluids. Lipase carries out transesterification reactions in the presence of supercritical carbon dioxide. Polyphenyl oxidase is active in supercritical CO{sub 2} and fluoroform. It has been shown that alkaline phosphatase and cholesterol oxidase are active in supercritical CO{sub 2}. More recently, an examination of the effect of aggregation of cholesterol on cholesterol oxidase activity in CO{sub 2} using electron paramagnetic resonance (EPR) was done. They found that when cosolvents which promoted aggregation were added, the reaction rate increased in proportion to the amount of aggregation. To date, no data on the effect of pressure on reaction rate have been presented. The objective of this work is to determine whether pressure-induced changes in the physical properties of a supercritical fluid solvent affect the rate of an enzymatic reaction and if so, which properties are responsible for the change.

• OSTI ID: 6775097
• Journal Information: A.I.Ch.E. Journal (American Institute of Chemical Engineers); (USA), Vol. 36:2; ISSN 0001-1541
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
37 INORGANIC
ORGANIC
PHYSICAL AND ANALYTICAL CHEMISTRY
75 CONDENSED MATTER PHYSICS
SUPERCONDUCTIVITY AND SUPERFLUIDITY
ENZYME ACTIVITY
PRESSURE DEPENDENCE
LIPASES
OXIDOREDUCTASES
SUPERCRITICAL STATE
BIOCHEMICAL REACTION KINETICS
CARBON DIOXIDE
CHEMICAL REACTION KINETICS
CRITICAL TEMPERATURE
RHIZOPUS
TRIGLYCERIDES
CARBON COMPOUNDS
CARBON OXIDES
CARBOXYLESTERASES
CHALCOGENIDES
ENZYMES
ESTERASES
ESTERS
EUMYCOTA
FUNGI
HYDROLASES
KINETICS
LIPIDS
ORGANIC COMPOUNDS
OXIDES
OXYGEN COMPOUNDS
PHYSICAL PROPERTIES
PLANTS
REACTION KINETICS
THERMODYNAMIC PROPERTIES
TRANSITION TEMPERATURE
550200* - Biochemistry
400201 - Chemical & Physicochemical Properties
640450 - Fluid Physics- Superfluidity