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Title: Photosynthetic regeneration of ATP using a strain of thermophilic blue-green algae

Abstract

Photosynthetic ATP accumulation was shown in the presence of exogenous ADP plus ortho-phosphate on illumination to the intact cells of a strain of thermophilic blue-green algae isolated from Matsue hot springs, Mastigocladus sp. Kinetic studies of various effectors on the ATP accumulation proved that the ATP synthesis depends mainly on the cyclic photophosphorylation system around photosystem I (PS-I) in the algal cells. The temperature and pH optima for the accumulation were found at 45 degrees C and pH 7.5. Maximum yield was obtained with light intensity higher than 15 mW/squared cm. Borate ion exerted pronounced enhancement on the ATP synthesis. With a continuous reactor at a flow rate of 1 ml/hour at 45 degrees C and pH 7.5, efficient photoconversion of ADP (2mM, at substrate reservoir) to ATP (1mM, at product outlet) has been maintained for a period of 2.5 days, though the efficiency has decreased in a further 2-day period to the level of 0.5 mM ATP/9.5 h of residence time. (Refs. 24).

Authors:
; ;
Publication Date:
Research Org.:
Lab Biochemistry, Coll Agriculture, Shimane Univ Nishikawazu-cho, Matsue, 690, Japan
OSTI Identifier:
6771571
Resource Type:
Journal Article
Journal Name:
Biotechnol. Bioeng.; (United States)
Additional Journal Information:
Journal Volume: 24:2
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 09 BIOMASS FUELS; 14 SOLAR ENERGY; ALGAE; ATP; CONTINUOUS CULTURE; BIOSYNTHESIS; ADP; CHLOROPLASTS; KINETICS; PH VALUE; PHOSPHORUS; PHOTOSYNTHESIS; TEMPERATURE EFFECTS; THERMOPHILIC CONDITIONS; CELL CONSTITUENTS; CHEMICAL REACTIONS; ELEMENTS; NONMETALS; NUCLEOTIDES; ORGANIC COMPOUNDS; PHOTOCHEMICAL REACTIONS; PLANTS; SYNTHESIS; 550700* - Microbiology; 140504 - Solar Energy Conversion- Biomass Production & Conversion- (-1989); 140505 - Solar Energy Conversion- Photochemical, Photobiological, & Thermochemical Conversion- (1980-)

Citation Formats

Sawa, Y, Kanayama, K, and Ochiai, H. Photosynthetic regeneration of ATP using a strain of thermophilic blue-green algae. United States: N. p., 1982. Web. doi:10.1002/bit.260240205.
Sawa, Y, Kanayama, K, & Ochiai, H. Photosynthetic regeneration of ATP using a strain of thermophilic blue-green algae. United States. https://doi.org/10.1002/bit.260240205
Sawa, Y, Kanayama, K, and Ochiai, H. 1982. "Photosynthetic regeneration of ATP using a strain of thermophilic blue-green algae". United States. https://doi.org/10.1002/bit.260240205.
@article{osti_6771571,
title = {Photosynthetic regeneration of ATP using a strain of thermophilic blue-green algae},
author = {Sawa, Y and Kanayama, K and Ochiai, H},
abstractNote = {Photosynthetic ATP accumulation was shown in the presence of exogenous ADP plus ortho-phosphate on illumination to the intact cells of a strain of thermophilic blue-green algae isolated from Matsue hot springs, Mastigocladus sp. Kinetic studies of various effectors on the ATP accumulation proved that the ATP synthesis depends mainly on the cyclic photophosphorylation system around photosystem I (PS-I) in the algal cells. The temperature and pH optima for the accumulation were found at 45 degrees C and pH 7.5. Maximum yield was obtained with light intensity higher than 15 mW/squared cm. Borate ion exerted pronounced enhancement on the ATP synthesis. With a continuous reactor at a flow rate of 1 ml/hour at 45 degrees C and pH 7.5, efficient photoconversion of ADP (2mM, at substrate reservoir) to ATP (1mM, at product outlet) has been maintained for a period of 2.5 days, though the efficiency has decreased in a further 2-day period to the level of 0.5 mM ATP/9.5 h of residence time. (Refs. 24).},
doi = {10.1002/bit.260240205},
url = {https://www.osti.gov/biblio/6771571}, journal = {Biotechnol. Bioeng.; (United States)},
number = ,
volume = 24:2,
place = {United States},
year = {Mon Feb 01 00:00:00 EST 1982},
month = {Mon Feb 01 00:00:00 EST 1982}
}