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Title: Intracellular phosphorylation of vitellogenin in the liver of estrogen-stimulated Xenopus laevis

Abstract

A procedure was developed for the preparation of rough and smooth microsomes from small quantities of liver obtained from estrogen-stimulated Xenopus laevis females. Morphological, biochemical, and enzymatic characterization of these fractions indicates that excellent separation of rough microsomes from smooth microsomes is achieved. In addition, pulse-chase experiments demonstrate that rough and smooth microsomes each exhibit the predictable patterns of incorporation characteristic of secretory protein synthesis and intracellular translocation. This procedure was combined with suitable incubation conditions for pulse-chase experiments which demonstrate the subcellular sites of vitellogenin phosphorylation. The data presented indicate that approximately 70% of the phosphate residues are covalently attached to vitellogenin during its intracellular translocation through the smooth microsomes, while the rough microsomes can account for the remainder of the total incorporated phosphate.

Authors:
;
Publication Date:
Research Org.:
Oak Ridge National Lab., TN
OSTI Identifier:
6771398
DOE Contract Number:  
W-7405-ENG-26
Resource Type:
Journal Article
Journal Name:
J. Biol. Chem.; (United States)
Additional Journal Information:
Journal Volume: 256:7
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; PROTEINS; PHOSPHORYLATION; BIOSYNTHESIS; ESTROGENS; FROGS; HISTOLOGICAL TECHNIQUES; LIVER; MICROSOMES; AMPHIBIANS; ANIMALS; AQUATIC ORGANISMS; BODY; CELL CONSTITUENTS; CHEMICAL REACTIONS; DIGESTIVE SYSTEM; GLANDS; HORMONES; ORGANIC COMPOUNDS; ORGANOIDS; ORGANS; STEROID HORMONES; SYNTHESIS; VERTEBRATES; 550201* - Biochemistry- Tracer Techniques

Citation Formats

Gottlieb, T A, and Wallace, R A. Intracellular phosphorylation of vitellogenin in the liver of estrogen-stimulated Xenopus laevis. United States: N. p., 1981. Web.
Gottlieb, T A, & Wallace, R A. Intracellular phosphorylation of vitellogenin in the liver of estrogen-stimulated Xenopus laevis. United States.
Gottlieb, T A, and Wallace, R A. 1981. "Intracellular phosphorylation of vitellogenin in the liver of estrogen-stimulated Xenopus laevis". United States.
@article{osti_6771398,
title = {Intracellular phosphorylation of vitellogenin in the liver of estrogen-stimulated Xenopus laevis},
author = {Gottlieb, T A and Wallace, R A},
abstractNote = {A procedure was developed for the preparation of rough and smooth microsomes from small quantities of liver obtained from estrogen-stimulated Xenopus laevis females. Morphological, biochemical, and enzymatic characterization of these fractions indicates that excellent separation of rough microsomes from smooth microsomes is achieved. In addition, pulse-chase experiments demonstrate that rough and smooth microsomes each exhibit the predictable patterns of incorporation characteristic of secretory protein synthesis and intracellular translocation. This procedure was combined with suitable incubation conditions for pulse-chase experiments which demonstrate the subcellular sites of vitellogenin phosphorylation. The data presented indicate that approximately 70% of the phosphate residues are covalently attached to vitellogenin during its intracellular translocation through the smooth microsomes, while the rough microsomes can account for the remainder of the total incorporated phosphate.},
doi = {},
url = {https://www.osti.gov/biblio/6771398}, journal = {J. Biol. Chem.; (United States)},
number = ,
volume = 256:7,
place = {United States},
year = {Fri Apr 10 00:00:00 EST 1981},
month = {Fri Apr 10 00:00:00 EST 1981}
}