Localization and stability of technetium-99m-Sn-pyrophosphate in rat neutrophils
Technetium-99m has been suggested as an alternative radiolabel for white cells, and while its physical characteristics are nearly ideal, its stability and site of localization in this procedure are unclear. We examined these parameters by radiolabeling 10(8) neutrophils from rat peritoneum with 74 to 370 MBq technetium-99m-Sn-pyrophosphate. We found that the percentage of initial activity bound to neutrophils was quite variable, possibly because the radiolabel associated with several subfractions: 19.8 +/- 11.5% (mean +/- s.d.) with nuclei and plasma membranes, 25.6 +/- 3.9% with mitochondria, 26.6 +/- 9.8% with microsomes, and 29.2 +/- 6.9% with cytosol. Approximately 80-90% of the radioactivity associated with neutrophils was not bound to protein and only about one-half of the activity localized to cell membranes was removable over 4 hr by pepsin digestion. We concluded that the variable labeling efficiency was due to the radiolabel's rather loose association with several cellular subfractions rather than specific binding to a unique substrate.
- Research Organization:
- Univ. of Colorado Health Sciences Center, Denver (USA)
- OSTI ID:
- 6770677
- Journal Information:
- J. Nucl. Med.; (United States), Vol. 29:8
- Country of Publication:
- United States
- Language:
- English
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NEUTROPHILS
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SENSITIVITY ANALYSIS
BIOLOGICAL LOCALIZATION
CELL MEMBRANES
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ISOMERIC NUCLEI
MICROSOMES
MITOCHONDRIA
PYROPHOSPHATES
RATS
STABILITY
TECHNETIUM 99
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
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INTERMEDIATE MASS NUCLEI
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
LABELLED COMPOUNDS
LEUKOCYTES
MAMMALS
MATERIALS
MEMBRANES
NUCLEI
ODD-EVEN NUCLEI
ORGANOIDS
OXYGEN COMPOUNDS
PHOSPHORUS COMPOUNDS
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RODENTS
TECHNETIUM ISOTOPES
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550601* - Medicine- Unsealed Radionuclides in Diagnostics