Metabolism of the /sup 18/O-methoxy substituent of 3-methoxybenzoic acid and other unlabeled methoxybenzoic acids by anaerobic bacteria. [Eubacterium limosum; Acetobacterium woodil; Syntrophococcus; Clostridium; Desulfotomaculum; Enterobacter]

Journal Article · Sun May 01 00:00:00 EDT 1988 · Appl. Environ. Microbiol.; (United States)

OSTI ID:6770242

DeWeerd, J A; Saxena, A; Nagle, Jr, D P; Sulflita, J M

The mechanism of the bioconversion of methoxylated benzoic acids to the hydroxylated derivatives was investigated with a model substrate and cultures of one anaerobic consortium, eight strict anaerobic bacteria, and one facultative anaerobic microorganism. We found that a haloaromatic dehalogenating consortium, a dehalogenating isolate from that consortium, Eubacterium, limosum, and a strain of Acetobacterium woodii metabolized 3-(methoxy-/sup 18/O)methoxybenzoic acid (3-anisic acid) to 3-(hydroxy-/sup 18/O)hydroxybenzoic acid stoichiometrically at rates of 1.5, 3.2, 52.4, and 36.7 nmol/min per mg of protein, respectively. A different strain of Acetobacterium and strains of Syntrophococcus, Clostridium Desulfotomaculum, Enterobacter, and an anaerobic bacterium, strain TH-001, were unable to transform this compound. The O-demethylating ability of E. limosum was induced only with appropriate methoxylated benzoates but not with D-glucose, lactate, isoleucine, or methanol. Cross-acclimation and growth experiments with E. limosum showed a rate of metabolism that was an order of magnitude slower and showed no growth with either 4-methoxysalicylic acid (2-hydroxy-4-methoxybenzoic acid) or 4-anisic acid (4-methoxybenzoic acid) when adapted to 3-anisic acid. However, A. woodii NZva-16 showed slower rates and no growth with 3- or 4-methoxysalicylic acid when adapted to 3-anisic acid in similar experiments.

• Research Organization: Univ. of Oklahoma, Norman (USA)
• OSTI ID: 6770242
• Journal Information: Appl. Environ. Microbiol.; (United States), Vol. 54:5
• Country of Publication: United States
• Language: English

Related Subjects

54 ENVIRONMENTAL SCIENCES
BACTERIA
METABOLISM
BENZOIC ACID
ANAEROBIC DIGESTION
ANAEROBIC CONDITIONS
ENZYME ACTIVITY
OXYGEN 18
BIOCONVERSION
CARBOXYLIC ACIDS
DIGESTION
EVEN-EVEN NUCLEI
ISOTOPES
LIGHT NUCLEI
MANAGEMENT
MICROORGANISMS
MONOCARBOXYLIC ACIDS
NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
OXYGEN ISOTOPES
PROCESSING
STABLE ISOTOPES
WASTE MANAGEMENT
WASTE PROCESSING
510200* - Environment
Terrestrial- Chemicals Monitoring & Transport- (-1989)