Cloning and physical mapping of RNA polymerase genes from Methanobacterium thermoautotrophicum and comparison of homologies and gene orders with those of RNA polymerase genes from other methanogenic archaebacteria

Journal Article · Sun May 01 00:00:00 EDT 1988 · J. Bacteriol.; (United States)

OSTI ID:6768894

Schallenberg, J; Moes, M; Truss, M; Reiser, W; Thomm, M; Stetter, K O; Klein, A

The structural genes encoding the four largest subunits of RNA polymerase, A, B', B'', and C, were physically mapped in Methanobacterium thermoautotrophicum Winter. The genes formed a cluster in the order B'', B', A, C and had a common orientation. DNA hybridization experiments yielded different degrees of homology between RNA polymerase gene sequences of different species of Methanobacterium and Methanococcus voltae. No homology was detectable between Methanobacterium thermoautotrophicum and Methanosarcina barkeri. From Southern hybridization experiments in which probes of the four genes from Methanobacterium thermoautotrophicum Winter and restriction digests of the genomic DNAs of the different methanogens were used, a common gene order of the RNA polymerase genes could be deduced.

• Research Organization: Philipps-Universitaet, Marburg/Lahn (West Germany)
• OSTI ID: 6768894
• Journal Information: J. Bacteriol.; (United States), Vol. 170:5
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
09 BIOMASS FUELS
METHANOGENIC BACTERIA
MOLECULAR BIOLOGY
RNA POLYMERASES
DNA SEQUENCING
DNA-CLONING
GENETIC MAPPING
COMPARATIVE EVALUATIONS
HYBRIDIZATION
BACTERIA
CLONING
ENZYMES
MAPPING
MICROORGANISMS
NUCLEOTIDYLTRANSFERASES
PHOSPHORUS-GROUP TRANSFERASES
POLYMERASES
STRUCTURAL CHEMICAL ANALYSIS
TRANSFERASES
550400* - Genetics
090122 - Hydrocarbon Fuels- Preparation from Wastes or Biomass- (1976-1989)