Potentiation of collagen synthesis in explants of the rabbit eye by 5 beta-dihydrocortisol

Journal Article · Mon Dec 01 00:00:00 EST 1986 · Invest. Ophthalmol. Visual Sci.; (United States)

OSTI ID:6764253

Southren, A L; Hernandez, M R; l'Hommedieu, D; Gordon, G G; Weinstein, B I

The biologic effect of 5 beta-dihydrocortisol on collagen synthesis was evaluated. The metabolite was found to potentiate subthreshold levels of dexamethasone in increasing /sup 3/H-proline incorporation in cells of the outflow region of the rabbit. Digestion of the tissue with highly purified collagenase indicated that the /sup 3/H-proline was incorporated into collagen type protein. This study demonstrates another biologic activity of 5 beta-dihydrocortisol, a metabolite found to accumulate in cells cultured from trabeculectomy specimens from patients with primary open angle glaucoma.

• Research Organization: New York Medical College, Valhalla
• OSTI ID: 6764253
• Journal Information: Invest. Ophthalmol. Visual Sci.; (United States), Vol. 12
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
COLLAGEN
BIOSYNTHESIS
EYES
TRANSPLANTS
HYDROCORTISONE
BIOLOGICAL EFFECTS
AUTORADIOGRAPHY
IN VITRO
PROLINE
RABBITS
TRITIUM COMPOUNDS
ADRENAL HORMONES
AMINES
AMINO ACIDS
ANIMALS
AZOLES
BODY
BODY AREAS
CARBOXYLIC ACIDS
CORTICOSTEROIDS
FACE
GLUCOCORTICOIDS
HEAD
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
HORMONES
HYDROXY COMPOUNDS
KETONES
LABELLED COMPOUNDS
MAMMALS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PREGNANES
PROTEINS
PYRROLES
PYRROLIDINES
SCLEROPROTEINS
SENSE ORGANS
STEROID HORMONES
STEROIDS
SYNTHESIS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques