Comparison of muscarine- and vasopressin-stimulated inositol phospholipid metabolism in the superior cervical ganglion of the rat

Conference · Wed Mar 05 00:00:00 EST 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:6762713

Horwitz, J; Anderson, C; Perlman, R L

Both muscarine and vasopressin have previously been shown to increase the accumulation of /sup 3/H-inositol phosphates (/sup 3/H-IP) in superior cervical ganglia in which the phospholipids were labeled with /sup 3/H-inositol. They have compared the effects of muscarine and vasopressin on phospholipid metabolism in the ganglion. The effects of these agents on /sup 3/H-IP accumulation are additive. The response to muscarine plateaus after approximately 10 min whereas the response to vasopressin increases for at least 30 min. Decentralization and maintenance in organ culture appear to potentiate the effect of muscarine on /sup 3/H-IP accumulation but do not effect the response of the ganglia to vasopressin. Muscarine and vasopressin also increase the incorporation of /sup 3/H-inositol into phospholipids in the ganglion. Autoradiographic techniques were used to localize the inositol-containing phospholipids in the ganglion. Muscarine increases phospholipid labeling primarily in the cell bodies of the principal ganglionic neurons, whereas vasopressin increases phospholipid labeling primarily in the neuropil. These data are consistent with the hypothesis that muscarine and vasopressin stimulate the metabolism of different pools of phospholipids.

• Research Organization: Illinois College of Medicine, Chicago
• OSTI ID: 6762713
• Report Number(s): CONF-8604222-; TRN: 87-010465
• Journal Information: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 45:4; Conference: 70. annual meeting of the Federation of American Society for Experimental Biology, St. Louis, MO, USA, 13 Apr 1986
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
CHOLINE
BIOCHEMICAL REACTION KINETICS
PHOSPHOLIPIDS
BIOLOGICAL LOCALIZATION
METABOLISM
VASOPRESSIN
AUTORADIOGRAPHY
BRAIN
COMPARATIVE EVALUATIONS
RATS
TRITIUM COMPOUNDS
ALCOHOLS
AMINES
AMMONIUM COMPOUNDS
ANIMALS
BODY
CENTRAL NERVOUS SYSTEM
DRUGS
ESTERS
HORMONES
HYDROXY COMPOUNDS
KINETICS
LABELLED COMPOUNDS
LIPIDS
LIPOTROPIC FACTORS
MAMMALS
NERVOUS SYSTEM
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
ORGANS
PEPTIDE HORMONES
PITUITARY HORMONES
QUATERNARY COMPOUNDS
REACTION KINETICS
RODENTS
VERTEBRATES
550501* - Metabolism- Tracer Techniques