Relative turnover of (/sup 3/H)arachidonic acid and (/sup 14/C)eicosapentaenoic acid in stimulated human platelets
Abstract
The relative release of arachidonic acid (AA) versus eicosapentaenoic acid (EPA) from platelet phospholipids may be important in accounting for the potential of dietary fish oil containing EPA to alter platelet reactivity. Human platelets enriched in EPA and prelabelled with (/sup 3/H)AA and (/sup 14/C)EPA were used to examine the relative losses of these fatty acids from platelet phospholipids upon stimulation. Washed dual-labelled platelets were incubated with and without thrombin in the presence of BW755C and in the presence and absence of trifluoperazine. The platelet lipids were extracted and the individual phospholipids as well as diacylglycerol (DG), phosphatidic acid (PA), etc. were separated by thin-layer chromatography and the radioactivity in each fraction determined. The (/sup 3/H)AA/(/sup 14/C)EPA dpm ratio for the loss in radioactivity from PC upon thrombin stimulation was similar to that for the PC in resting platelets. This suggests no marked selectivity in the degradation of AA versus EPA species of PC during platelet activation. The (/sup 3/H)/(/sup 14/C) ratios for the increased radioactivity in DG and PA upon thrombin stimulation were slightly higher than the ratio in PI from resting platelets suggesting only a minor preference for 1-acyl 2-arachidonoyl PI over 1-acyl 2-eicosapentaenoyl PI in the pathwaymore »
- Authors:
- Publication Date:
- Research Org.:
- Univ. of Guelph, Ontario
- OSTI Identifier:
- 6762664
- Report Number(s):
- CONF-8604222-
Journal ID: CODEN: FEPRA; TRN: 87-010471
- Resource Type:
- Conference
- Journal Name:
- Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)
- Additional Journal Information:
- Journal Volume: 45:4; Conference: 70. annual meeting of the Federation of American Society for Experimental Biology, St. Louis, MO, USA, 13 Apr 1986
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; ARACHIDONIC ACID; METABOLISM; EICOSANOIC ACID; BIOLOGICAL PATHWAYS; BLOOD PLATELETS; CARBON 14 COMPOUNDS; FISHES; MAN; OILS; PHOSPHOLIPIDS; THIN-LAYER CHROMATOGRAPHY; TRACER TECHNIQUES; TRITIUM COMPOUNDS; ANIMALS; AQUATIC ORGANISMS; BIOLOGICAL MATERIALS; BLOOD; BLOOD CELLS; BODY FLUIDS; CARBOXYLIC ACIDS; CHROMATOGRAPHY; ESTERS; ISOTOPE APPLICATIONS; LABELLED COMPOUNDS; LIPIDS; MAMMALS; MATERIALS; MONOCARBOXYLIC ACIDS; ORGANIC ACIDS; ORGANIC COMPOUNDS; ORGANIC PHOSPHORUS COMPOUNDS; OTHER ORGANIC COMPOUNDS; PRIMATES; SEPARATION PROCESSES; VERTEBRATES; 550501* - Metabolism- Tracer Techniques
Citation Formats
Weaver, B J, and Holub, B J. Relative turnover of (/sup 3/H)arachidonic acid and (/sup 14/C)eicosapentaenoic acid in stimulated human platelets. United States: N. p., 1986.
Web.
Weaver, B J, & Holub, B J. Relative turnover of (/sup 3/H)arachidonic acid and (/sup 14/C)eicosapentaenoic acid in stimulated human platelets. United States.
Weaver, B J, and Holub, B J. 1986.
"Relative turnover of (/sup 3/H)arachidonic acid and (/sup 14/C)eicosapentaenoic acid in stimulated human platelets". United States.
@article{osti_6762664,
title = {Relative turnover of (/sup 3/H)arachidonic acid and (/sup 14/C)eicosapentaenoic acid in stimulated human platelets},
author = {Weaver, B J and Holub, B J},
abstractNote = {The relative release of arachidonic acid (AA) versus eicosapentaenoic acid (EPA) from platelet phospholipids may be important in accounting for the potential of dietary fish oil containing EPA to alter platelet reactivity. Human platelets enriched in EPA and prelabelled with (/sup 3/H)AA and (/sup 14/C)EPA were used to examine the relative losses of these fatty acids from platelet phospholipids upon stimulation. Washed dual-labelled platelets were incubated with and without thrombin in the presence of BW755C and in the presence and absence of trifluoperazine. The platelet lipids were extracted and the individual phospholipids as well as diacylglycerol (DG), phosphatidic acid (PA), etc. were separated by thin-layer chromatography and the radioactivity in each fraction determined. The (/sup 3/H)AA/(/sup 14/C)EPA dpm ratio for the loss in radioactivity from PC upon thrombin stimulation was similar to that for the PC in resting platelets. This suggests no marked selectivity in the degradation of AA versus EPA species of PC during platelet activation. The (/sup 3/H)/(/sup 14/C) ratios for the increased radioactivity in DG and PA upon thrombin stimulation were slightly higher than the ratio in PI from resting platelets suggesting only a minor preference for 1-acyl 2-arachidonoyl PI over 1-acyl 2-eicosapentaenoyl PI in the pathway from PI to DG to PA.},
doi = {},
url = {https://www.osti.gov/biblio/6762664},
journal = {Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)},
number = ,
volume = 45:4,
place = {United States},
year = {Wed Mar 05 00:00:00 EST 1986},
month = {Wed Mar 05 00:00:00 EST 1986}
}