Diffusion-enhanced lanthanide energy transfer studies of protein prosthetic groups

Journal Article · Wed Oct 01 00:00:00 EDT 1980 · Biophys. J.; (United States)

DOI:https://doi.org/10.1016/S0006-3495(80)84942-3· OSTI ID:6761272

Meares, C F; Yeh, S M; Rice, L S

A long-lived luminescent solute in aqueous solution (e.g., /sup 5/D/sub 4/ terbium, tau approx. =10/sup -3/s) can donate its excitation energy to a chromophore such as a protein prosthetic group by, e.g., the radiationless dipolar mechanism of Foerster. However, in contrast to the usual energy-transfer experiment, a donor with a 10/sup -3/s lifetime can diffuse extensively through the solution and, in a time scale short compared to its excited lifetime, sample all permitted locations with respect to chromophoric acceptors. As recently indicated by Thomas et al. energy transfer in this rapid-diffusion limit can permit direct measurement of the allowed distance of closest approach of small solute molecules to chromophores which may be buried within proteins or membranes.

• Research Organization: Univ. of California, Davis, CA (United States)
• OSTI ID: 6761272
• Journal Information: Biophys. J.; (United States), Vol. 32:1
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
PROTEINS
STRUCTURAL CHEMICAL ANALYSIS
AQUEOUS SOLUTIONS
BIOCHEMICAL REACTION KINETICS
DIFFUSION
ENERGY TRANSFER
LUMINESCENCE
MOLECULAR STRUCTURE
RARE EARTHS
DISPERSIONS
ELEMENTS
KINETICS
METALS
MIXTURES
ORGANIC COMPOUNDS
REACTION KINETICS
SOLUTIONS
550200* - Biochemistry