Cloning and linkage mapping of three polymorphic tetranucleotide (TAAA)[sub n] repeats on human chromosome 21

Journal Article · Tue Dec 01 00:00:00 EST 1992 · Genomics; (United States)

DOI:https://doi.org/10.1016/S0888-7543(05)80131-4· OSTI ID:6758473

Kalaitsidaki, M; Antonarakis, S E ^[1]; Cox, T; Chakravarti, A ^[2]

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1. Johns Hopkins Univ. School of Medicine, Baltimore, MD (United States)
2. Univ. of Pittsburgh, PA (United States)

The authors report the cloning, sequencing, and mapping of three short sequence repeat polymorphisms due to tetranucleotide (TAAA) repeats from human chromosome 21. These DNA markers (D21S221, D21S225, D21S226) have been cloned from the chromosome 21-specific plasmid library of J. C. Fuscoe, C. C. Collins, D. Pinkel, and J. W. Gray and were shown to be polymorphic by polymerase chain reaction amplification and polyacrylamide gel electrophoresis. Genotypes were determined in informative CEPH pedigrees and used in linkage analysis relative to other mapped markers on human chromosome 21. One of these markers, D21S221, is closely linked to the amyloid precursor protein gene (APP), which has been implicated in the etiology of familial Alzheimer disease in some families. 18 refs., 3 figs., 2 tabs.

• DOE Contract Number: FG02-89ER60857
• OSTI ID: 6758473
• Journal Information: Genomics; (United States), Vol. 14:4; ISSN 0888-7543
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
BIOLOGICAL MARKERS
GENETIC MAPPING
HUMAN CHROMOSOME 21
BRAIN
DNA SEQUENCING
DNA-CLONING
NERVOUS SYSTEM DISEASES
BIOTECHNOLOGY
BODY
CENTRAL NERVOUS SYSTEM
CHROMOSOMES
CLONING
DISEASES
DNA HYBRIDIZATION
GENETIC ENGINEERING
HUMAN CHROMOSOMES
HYBRIDIZATION
MAPPING
NERVOUS SYSTEM
NUCLEIC ACID HYBRIDIZATION
ORGANS
STRUCTURAL CHEMICAL ANALYSIS
550400* - Genetics