Serially transplantable chemically induced rat islet cell tumor

Journal Article · Wed Oct 01 00:00:00 EDT 1980 · Endocrinology; (United States)

DOI:https://doi.org/10.1210/endo-107-4-954· OSTI ID:6756902

Chick, W L ^[1]; Appel, M C; Weir, G C; Like, A A; Lauris, V; Porter, J G; Chute, R N

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1. Harvard Medical School, Boston, MA

A serially transplantable, chemically induced pancreatic islet cell tumor was developed in Lewis rats. The original tumor was induced by the administration of streptozotocin and nicotinamide. It was subsequently maintained by ip or sc transplantation of tissue fragments into recipient animals. They were well encapsulated, without gross evidence of metastasis. Peroxidase immunocytochemical staining revealed a predominance of insulin-positive cells. Somatostatin-positive cells were also present and varied widely in numbers between different tumors. On electron microscopy, cells containing secretory granules, indistinguishable from nonneoplastic ..beta..-cells, were most abundant. Tumor extracts contained an average of 3260 ..mu..g insulin, 22.6 ..mu..g somatostatin, and 0.84 ..mu..g glucagon per g wet wt of tissue.

• OSTI ID: 6756902
• Journal Information: Endocrinology; (United States), Vol. 107:4
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
INSULIN
BIOLOGICAL EFFECTS
NEOPLASMS
MORPHOLOGY
TRANSMISSION ELECTRON MICROSCOPY
TRANSPLANTS
SOMATOSTATIN
NICOTINAMIDE
NITROSO COMPOUNDS
PANCREAS
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DIGESTIVE SYSTEM
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HETEROCYCLIC COMPOUNDS
HORMONES
MAMMALS
MICROSCOPY
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PEPTIDE HORMONES
PYRIDINES
RODENTS
VERTEBRATES
VITAMIN B GROUP
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560305* - Chemicals Metabolism & Toxicology- Vertebrates- (-1987)