Serially transplantable chemically induced rat islet cell tumor
- Harvard Medical School, Boston, MA
A serially transplantable, chemically induced pancreatic islet cell tumor was developed in Lewis rats. The original tumor was induced by the administration of streptozotocin and nicotinamide. It was subsequently maintained by ip or sc transplantation of tissue fragments into recipient animals. They were well encapsulated, without gross evidence of metastasis. Peroxidase immunocytochemical staining revealed a predominance of insulin-positive cells. Somatostatin-positive cells were also present and varied widely in numbers between different tumors. On electron microscopy, cells containing secretory granules, indistinguishable from nonneoplastic ..beta..-cells, were most abundant. Tumor extracts contained an average of 3260 ..mu..g insulin, 22.6 ..mu..g somatostatin, and 0.84 ..mu..g glucagon per g wet wt of tissue.
- OSTI ID:
- 6756902
- Journal Information:
- Endocrinology; (United States), Vol. 107:4
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
INSULIN
BIOLOGICAL EFFECTS
NEOPLASMS
MORPHOLOGY
TRANSMISSION ELECTRON MICROSCOPY
TRANSPLANTS
SOMATOSTATIN
NICOTINAMIDE
NITROSO COMPOUNDS
PANCREAS
RATS
SACCHARIDES
AMIDES
ANIMALS
AZINES
BODY
CARBOHYDRATES
DIGESTIVE SYSTEM
DISEASES
ELECTRON MICROSCOPY
ENDOCRINE GLANDS
GLANDS
HETEROCYCLIC COMPOUNDS
HORMONES
MAMMALS
MICROSCOPY
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PEPTIDE HORMONES
PYRIDINES
RODENTS
VERTEBRATES
VITAMIN B GROUP
VITAMINS
560305* - Chemicals Metabolism & Toxicology- Vertebrates- (-1987)