Neutrophil chemotactic factor release and neutrophil alveolitis in asbestos-exposed individuals

Journal Article · Thu Sep 01 00:00:00 EDT 1988 · Chest; (United States)

DOI:https://doi.org/10.1378/chest.94.3.521· OSTI ID:6754676

Hayes, A A; Rose, A H; Musk, A W; Robinson, B W

Alveolar neutrophil accumulation occurs in asbestosis. To evaluate a possible role for release of neutrophil chemotactic factor (NCF) in the pathogenesis of asbestosis, spontaneous NCF release from alveolar macrophages obtained by bronchoalveolar lavage (BAL) in eight individuals with asbestosis, 13 asbestos-exposed individuals without asbestosis, and five control subjects has been studied. Alveolar macrophages were incubated in medium (four hours; 37 degrees C), and neutrophil responses to the supernatants were assayed in a microchemotaxis chamber. Alveolar macrophages from subjects with asbestosis released more NCF (97 +/- 19 neutrophils per high-power field (N/HPF)) than controls (3 +/- 1 N/HPF; p less than 0.01). Alveolar macrophages from individuals with asbestos exposure and increased BAL neutrophil proportions (n = 7) released more NCF (93 +/- 24 N/HPF) than individuals with asbestos exposure and normal BAL neutrophil proportions (n = 6; 11 +/- 6 N/HPF; p less than 0.02). The results show that spontaneous NCF release occurs in asbestosis and that NCF release is associated with neutrophil alveolitis in asbestos-exposed individuals without asbestosis, suggesting a pathogenic role for NCF in mediating this neutrophil alveolitis. The results of the study also suggest that the presence of crackles is a better predictor of the presence of neutrophil alveolitis than is an abnormal chest x-ray film.

• Research Organization: Queen Elizabeth II Medical Centre, Nedlands (Western Australia)
• OSTI ID: 6754676
• Journal Information: Chest; (United States), Vol. 94:3
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
59 BASIC BIOLOGICAL SCIENCES
ASBESTOS
TOXICITY
NEUTROPHILS
SECRETION
RESPIRATORY SYSTEM DISEASES
PATHOGENESIS
ENVIRONMENTAL EXPOSURE
INFLAMMATION
LAVAGE
MACROPHAGES
ANIMAL CELLS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CONNECTIVE TISSUE CELLS
DISEASES
LEUKOCYTES
MATERIALS
PATHOLOGICAL CHANGES
PHAGOCYTES
SOMATIC CELLS
SYMPTOMS
560300* - Chemicals Metabolism & Toxicology
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