Effects of argon laser on in vitro aggregation of platelets in platelet rich plasma and whole blood

Journal Article · Wed Jun 01 00:00:00 EDT 1988 · Thromb. Res.; (United States)

DOI:https://doi.org/10.1016/0049-3848(88)90324-6· OSTI ID:6753962

Doerger, P T; Glueck, H I; McGill, M

The effects of an Argon laser on platelet aggregation were studied, since platelets may be exposed to laser energy when used intravascularly. Various preparations of platelets in platelet rich plasma (PRP) and whole blood, with or without aspirin, were tested with the aggregating agents ADP, collagen, thrombin, and epinephrine. Simultaneous release of ATP was also measured in PRP. At relatively low levels of irradiation, platelet aggregation was potentiated. Enhancement was evidenced by an increase in percent aggregation, earlier onset of the reaction, and reduction in the amount of aggregating agent required. In PRP, the mechanism of laser potentiation appeared to be the release of endogenous ATP from platelets. At relatively high levels of irradiation, platelets were destroyed and aggregation abolished. In whole blood, the mechanism was somewhat more complicated since release of ATP occurred from RBCs as well as platelets. Spontaneous aggregation following laser treatment occurred in isolated instances in PRP and in every trial in whole blood preparations. Aspirin ingestion inhibited the laser's effects in PRP but not in whole blood. These results may have important clinical implications for laser angioplasty, and the potentiated aggregation response may prove useful in laboratory studies of platelet function.

• Research Organization: Univ. of Cincinnati College of Medicine, OH (USA)
• OSTI ID: 6753962
• Journal Information: Thromb. Res.; (United States), Vol. 50:5
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BLOOD PLATELETS
BLOOD COAGULATION
LASER RADIATION
BIOLOGICAL EFFECTS
ADP
ADRENALINE
ATP
BLOOD
COLLAGEN
GAS LASERS
IN VITRO
SALICYLIC ACID
THROMBIN
ADRENAL HORMONES
AUTONOMIC NERVOUS SYSTEM AGENTS
BIOLOGICAL MATERIALS
BLOOD CELLS
BODY FLUIDS
CARBOXYLIC ACIDS
CARDIOTONICS
CARDIOVASCULAR AGENTS
COAGULANTS
DRUGS
ELECTROMAGNETIC RADIATION
ENZYMES
HEMATOLOGIC AGENTS
HEMOSTATICS
HYDROLASES
HYDROXY ACIDS
LASERS
MATERIALS
NEUROREGULATORS
NUCLEOTIDES
ORGANIC ACIDS
ORGANIC COMPOUNDS
PEPTIDE HYDROLASES
PROTEINS
RADIATIONS
SCLEROPROTEINS
SERINE PROTEINASES
SYMPATHOMIMETICS
560400* - Other Environmental Pollutant Effects