Role of platelets in maintenance of pulmonary vascular permeability to protein

Journal Article · Fri Apr 01 00:00:00 EST 1988 · American Journal of Physiology; (USA)

OSTI ID:6749464

Lo, S K; Burhop, K E; Kaplan, J E; Malik, A B ^[1]

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1. Albany Medical College of Union Univ., NY (USA)

The authors examined the role of platelets in maintenance of pulmonary vascular integrity by inducing thrombocytopenia in sheep using antiplatelet serum (APS). A causal relationship between thrombocytopenia and increase in pulmonary vascular permeability was established by platelet repletion using platelet-rich plasma (PRP). Sheep were chronically instrumented and lung lymph fistulas prepared to monitor pulmonary lymph flow (Q{sub lym}). A balloon catheter was positioned in the left atrium to assess pulmonary vascular permeability to protein after raising the left atrial pressure (P{sub la}). Thrombocytopenia was maintained for 3 days by daily intramuscular APS injections. In studies using cultured bovine pulmonary artery endothelial monolayers, transendothelia permeability of {sup 125}I-labeled albumin was reduced 50 and 95%, respectively, when 2.5 {times} 10{sup 7} or 5 {times} 10{sup 7} platelets were added onto endothelial monolayers. However, addition of 5 {times} 10{sup 6} platelets or 5 {times} 10{sup 7} red blood cells did not reduce endothelial monolayer albumin permeability. Results indicate that platelets are required for the maintenance of pulmonary vascular permeability. Reduction in permeability appears to involve an interaction of platelets with the endothelium.

• OSTI ID: 6749464
• Journal Information: American Journal of Physiology; (USA), Vol. 254:4; ISSN 0002-9513
• Country of Publication: United States
• Language: English

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