Specific binding of lactoferrin to brush-border membrane: Ontogeny and effect of glycan chain

Journal Article · Fri Apr 01 00:00:00 EST 1988 · American Journal of Physiology; (USA)

OSTI ID:6749084

Davidson, L A; Loennerdal, B ^[1]

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1. Univ. of California, Davis (USA)

Bioavailability of iron from human milk is exceptionally high. It has been suggested that lactoferrin, the major iron-binding protein in human milk, may participate in this high iron bioavailability from milk. The authors examined the interaction of lactoferrin with the intestinal brush-border membrane using the rhesus monkeys as a model. Brush-border membrane vesicles were prepared from monkeys of various ages. Binding studies with {sup 59}Fe-labeled human and monkey lactoferrin were performed to examine interaction of lactoferrin with the brush-border membrane. Specific saturable binding of lactoferrin was found at all ages studied. The dissociation constant for lactoferrin-receptor binding was 9 {times} 10{sup {minus}6} M. In contrast, no binding of serum transferrin or bovine lactoferrin occurred. Removal of fucose from the lactoferrin glycans resulted in a significant decrease in binding. It was concluded that lactoferrin in milk may function in the process of iron absorption through interaction with a small intestinal receptor and that fucosylated glycans on the carbohydrate chain of lactoferrin are necessary for receptor recognition.

• OSTI ID: 6749084
• Journal Information: American Journal of Physiology; (USA), Vol. 254:4; ISSN 0002-9513
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
IRON 59
CROSS-LINKING
LACTOFERRIN
INTESTINAL ABSORPTION
MEMBRANE TRANSPORT
AGE DEPENDENCE
RECEPTORS
ADULTS
BIOCHEMICAL REACTION KINETICS
IRON CHLORIDES
JUVENILES
LABELLED COMPOUNDS
MEMBRANES
MILK
MONKEYS
NEONATES
ABSORPTION
AGE GROUPS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BODY FLUIDS
CARBOHYDRATES
CHEMICAL REACTIONS
CHLORIDES
CHLORINE COMPOUNDS
DAYS LIVING RADIOISOTOPES
EVEN-ODD NUCLEI
FOOD
GLOBULINS
GLUCOPROTEINS
GLYCOPROTEINS
HALIDES
HALOGEN COMPOUNDS
INTERMEDIATE MASS NUCLEI
IRON COMPOUNDS
IRON ISOTOPES
ISOTOPES
KINETICS
MAMMALS
MATERIALS
MEMBRANE PROTEINS
METALLOPROTEINS
NUCLEI
ORGANIC COMPOUNDS
POLYMERIZATION
PRIMATES
PROTEINS
RADIOISOTOPES
REACTION KINETICS
SACCHARIDES
TRANSITION ELEMENT COMPOUNDS
UPTAKE
VERTEBRATES
551001* - Physiological Systems- Tracer Techniques