Involvement of DNA polymerase alpha in host cell reactivation of UV-irradiated herpes simplex virus
Aphidicolin is a potent inhibitor of both host cell DNA polymerase alpha and herpes simplex virus (HSV)-induced DNA polymerase but has no effect on DNA polymerases beta and gamma of host cells. By using an aphidicolin-resistant mutant (Aphr) of HSV, a possible involvement of DNA polymerase alpha in host cell reactivation of UV-damaged HSV was studied. Plaque formation by UV-irradiated Aphr was markedly inhibited by 1 microgram of aphidicolin per ml, which did not affect the plating efficiency of nonirradiated Aphr. Aphidicolin added before 12 h postinfection inhibited plaque formation by irradiated Aphr, which became aphidicolin insensitive after 36 h postinfection. The results strongly suggest that host cell DNA polymerase alpha is involved in the repair of UV-irradiated HSV DNA.
- Research Organization:
- Nagoya Univ. School of Medicine, Japan
- OSTI ID:
- 6748637
- Journal Information:
- J. Virol.; (United States), Vol. 49:2
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
HERPES SIMPLEX
HOST-CELL REACTIVATION
POLYMERASES
ENZYME ACTIVITY
ENZYME INHIBITORS
INHIBITION
PLAQUE FORMATION
ULTRAVIOLET RADIATION
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
DISEASES
ELECTROMAGNETIC RADIATION
ENZYMES
INFECTIOUS DISEASES
NUCLEOTIDYLTRANSFERASES
PHOSPHORUS-GROUP TRANSFERASES
RADIATIONS
RECOVERY
REPAIR
SKIN DISEASES
TRANSFERASES
VIRAL DISEASES
560131* - Radiation Effects on Microorganisms- Basic Studies- (-1987)