Biochemical studies on the toxicity of hematite dust. [Guinea pigs]
Biochemical alterations in guinea pig lungs caused by hematite dust were followed at 150 days after intratracheal administration of the dust. In vivo dust exposure caused a significant increase in mitochondrial protein content and cytochrome c oxidase activity whereas diaphorase activity remained unaltered. Mitochondria from the exposed animals were apparently in a swollen state and their contraction profile upon the addition of ATP reflected permeability changes. However, in vitro dust caused no significant alterations. Significant increases in glycogen content along with an insignificant decrease in glycogen phosphorylase activity were also observed in hematite-treated guinea pig lungs. Decrease in drug-metabolizing enzymes such as aniline hydroxylase and tyrosine aminotransferase activities were also evident in the post mitochondrial fraction of the siderotic lungs. (/sup 3/H)Leucine-incorporation studies showed increased protein synthesis in the postmitochondrial fraction. Increase in protein synthesis in mitochondria was only marginal whereas in whole homogenate it decreased considerably. Experiments employing dust tagged with radioactive iron indicated the rapid mobilization of iron from lung and its distribution to various organs. The presence of iron-binding protein was confirmed by employing Sephadex gel-filtration techniques.
- Research Organization:
- Industrial Toxicology Research Centre, Lucknow, India
- OSTI ID:
- 6748110
- Journal Information:
- Environ. Res.; (United States), Vol. 32:2
- Country of Publication:
- United States
- Language:
- English
Similar Records
Biochemical effects of selenium and cadmium on the guinea pig lung following intratracheal instillation
HISTOCHEMICAL EXAMINATIONS OF THE BEHAVIOR OF SOME ENZYMES IN THE LUNG IN ACUTE RADIATION SICKNESS AS WELL AS AFTER LOCAL X IRRADIATION
Related Subjects
DUSTS
INTRATRACHEAL ADMINISTRATION
TOXICITY
ENZYME ACTIVITY
INHIBITION
HEMATITE
IRON
TISSUE DISTRIBUTION
MITOCHONDRIA
PATHOLOGICAL CHANGES
BIOLOGICAL EFFECTS
GUINEA PIGS
LABELLED COMPOUNDS
LUNGS
TRITIUM COMPOUNDS
ANIMALS
BODY
CELL CONSTITUENTS
CHALCOGENIDES
DISTRIBUTION
ELEMENTS
IRON COMPOUNDS
IRON ORES
IRON OXIDES
MAMMALS
METALS
MINERALS
ORES
ORGANOIDS
ORGANS
OXIDE MINERALS
OXIDES
OXYGEN COMPOUNDS
RESPIRATORY SYSTEM
RODENTS
TRANSITION ELEMENT COMPOUNDS
TRANSITION ELEMENTS
VERTEBRATES
560305* - Chemicals Metabolism & Toxicology- Vertebrates- (-1987)