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Title: Chemically induced transplantable malignant fibrous histiocytoma of the rat. Analyses with immunohistochemistry, immunoelectron microscopy and (3H)thymidine autoradiography

Abstract

Malignant fibrous histiocytoma was produced in rats by injection of 9,10-dimethyl-1,2-benzanthracene into their knee joints. The original tumors consisted mainly of fibroblast-like cells and histiocyte-like cells, often intermixed with bizarre giant cells, and they frequently showed the storiform-pleomorphic pattern. By immunohistochemistry, anti-rat macrophage monoclonal antibodies, TRPM-3, RM-1, and Ki-M2R, and anti-rat leukocyte common antigen reacted to the histiocyte-like cells but not to the fibroblast-like cells. By the single cell cloning method, we established six tumor cell lines, none of which reacted with the anti-rat macrophage monoclonal antibodies, possessed any Fc receptors, or conducted immune phagocytosis and Latex particle phagocytosis. The ultrastructure of the cloned tumor cells resembled that of long-term cultured dermal fibroblasts. Collagen production by the tumor cells was demonstrated immunohistochemically with a monoclonal antibody for type I collagen. Inoculation of the cloned tumor cells into rats produced tumors with the histology of malignant fibrous histiocytoma and induced prominent macrophage infiltration. In the rat tumors produced by the inoculation of (3H)thymidine labeled cells, no reactivity of tumor cells with the anti-rat macrophage monoclonal antibodies was observed. Transplantation of the cultured rat tumor cells into nude mice produced tumors similar in histology to the original rat malignant fibrous histiocytoma. Tumormore » cells in nude mice induced marked macrophage infiltration as detected by immunohistochemistry with the anti-mouse macrophage monoclonal antibody F4/80. No differentiation of tumor cells into macrophages was detected, since no cells were stained with biotinylated anti-rat macrophage monoclonal antibody TRPM-3.« less

Authors:
; ; ;  [1]
  1. Kumamoto Univ. Medical School (Japan)
Publication Date:
OSTI Identifier:
6747775
Resource Type:
Journal Article
Journal Name:
Laboratory Investigation (A Journal of Experimental Methods and Pathology); (USA)
Additional Journal Information:
Journal Volume: 62:5; Journal ID: ISSN 0023-6837
Country of Publication:
United States
Language:
English
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; 59 BASIC BIOLOGICAL SCIENCES; BENZANTHRACENE; CARCINOGENESIS; TUMOR CELLS; TRANSPLANTS; AUTORADIOGRAPHY; CLONING; COLLAGEN; ELECTRON MICROSCOPY; EXPERIMENTAL NEOPLASMS; MACROPHAGES; MICE; MONOCLONAL ANTIBODIES; RATS; THYMIDINE; TRITIUM COMPOUNDS; ANIMAL CELLS; ANIMALS; ANTIBODIES; AROMATICS; AZINES; CONDENSED AROMATICS; CONNECTIVE TISSUE CELLS; HETEROCYCLIC COMPOUNDS; HYDROCARBONS; HYDROGEN COMPOUNDS; MAMMALS; MICROSCOPY; NUCLEOSIDES; NUCLEOTIDES; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; PATHOGENESIS; PHAGOCYTES; PROTEINS; PYRIMIDINES; RIBOSIDES; RODENTS; SCLEROPROTEINS; SOMATIC CELLS; VERTEBRATES; 560300* - Chemicals Metabolism & Toxicology; 550901 - Pathology- Tracer Techniques

Citation Formats

Kato, T, Takeya, M, Takagi, K, and Takahashi, K. Chemically induced transplantable malignant fibrous histiocytoma of the rat. Analyses with immunohistochemistry, immunoelectron microscopy and (3H)thymidine autoradiography. United States: N. p., 1990. Web.
Kato, T, Takeya, M, Takagi, K, & Takahashi, K. Chemically induced transplantable malignant fibrous histiocytoma of the rat. Analyses with immunohistochemistry, immunoelectron microscopy and (3H)thymidine autoradiography. United States.
Kato, T, Takeya, M, Takagi, K, and Takahashi, K. 1990. "Chemically induced transplantable malignant fibrous histiocytoma of the rat. Analyses with immunohistochemistry, immunoelectron microscopy and (3H)thymidine autoradiography". United States.
@article{osti_6747775,
title = {Chemically induced transplantable malignant fibrous histiocytoma of the rat. Analyses with immunohistochemistry, immunoelectron microscopy and (3H)thymidine autoradiography},
author = {Kato, T and Takeya, M and Takagi, K and Takahashi, K},
abstractNote = {Malignant fibrous histiocytoma was produced in rats by injection of 9,10-dimethyl-1,2-benzanthracene into their knee joints. The original tumors consisted mainly of fibroblast-like cells and histiocyte-like cells, often intermixed with bizarre giant cells, and they frequently showed the storiform-pleomorphic pattern. By immunohistochemistry, anti-rat macrophage monoclonal antibodies, TRPM-3, RM-1, and Ki-M2R, and anti-rat leukocyte common antigen reacted to the histiocyte-like cells but not to the fibroblast-like cells. By the single cell cloning method, we established six tumor cell lines, none of which reacted with the anti-rat macrophage monoclonal antibodies, possessed any Fc receptors, or conducted immune phagocytosis and Latex particle phagocytosis. The ultrastructure of the cloned tumor cells resembled that of long-term cultured dermal fibroblasts. Collagen production by the tumor cells was demonstrated immunohistochemically with a monoclonal antibody for type I collagen. Inoculation of the cloned tumor cells into rats produced tumors with the histology of malignant fibrous histiocytoma and induced prominent macrophage infiltration. In the rat tumors produced by the inoculation of (3H)thymidine labeled cells, no reactivity of tumor cells with the anti-rat macrophage monoclonal antibodies was observed. Transplantation of the cultured rat tumor cells into nude mice produced tumors similar in histology to the original rat malignant fibrous histiocytoma. Tumor cells in nude mice induced marked macrophage infiltration as detected by immunohistochemistry with the anti-mouse macrophage monoclonal antibody F4/80. No differentiation of tumor cells into macrophages was detected, since no cells were stained with biotinylated anti-rat macrophage monoclonal antibody TRPM-3.},
doi = {},
url = {https://www.osti.gov/biblio/6747775}, journal = {Laboratory Investigation (A Journal of Experimental Methods and Pathology); (USA)},
issn = {0023-6837},
number = ,
volume = 62:5,
place = {United States},
year = {Tue May 01 00:00:00 EDT 1990},
month = {Tue May 01 00:00:00 EDT 1990}
}