Protease production by immobilized mycelia of Streptomyces fradiae

Journal Article · Thu Jan 01 00:00:00 EST 1981 · Biotechnol. Bioeng.; (United States)

DOI:https://doi.org/10.1002/bit.260230104· OSTI ID:6747621

Kokubu, T; Karube, I; Suzuki, S

Streptomyces fradiae was immobilized in polyacrylamide gel prepared from 5% total acrylamide (90% acrylamide and 10% N,N-methylenebisacrylamide). Production of protease by the immobilized mycelia was attempted in a batch system. A dilute medium containing 0.5% starch, 0.5% meat extract, and 0.5% yeast extract was employed. The reusability of the immobilized and washed mycelia was examined. The activity of protease production by washed mycelia was rapidly decreased with increasing use cycles. The activity of the immobilized mycelia increased gradually, and reached a maximum after ten use cycles. Then, the activity gradually decreased with increasing reaction cycles. This might be caused by destruction of the gels. On the other hand, the sterilization of the surface of the immobilized mycelia was effective for elongation of the lifetime. As a result, the half-life of protease production by the sterilized immobilized mycelia was about 30 days. The rate of protease production by immobilized mycelia was 12,000 U/ml/hr. This value was four times higher than that by submerged culture.

• Research Organization: Dept Industrial Chemistry, Miyakonojo Coll of Technology, 473-1, Yoshiocho, Miyakonojo, 885, Miyazaki, Japan
• OSTI ID: 6747621
• Journal Information: Biotechnol. Bioeng.; (United States), Vol. 23:1
• Country of Publication: United States
• Language: English

Related Subjects

09 BIOMASS FUELS
59 BASIC BIOLOGICAL SCIENCES
HYDROLASES
BIOSYNTHESIS
IMMOBILIZED CELLS
ENZYME ACTIVITY
BACILLUS SUBTILIS
BATCH CULTURE
CULTURE MEDIA
ENZYMES
FERMENTATION
PROTEINS
STREPTOMYCES
BACILLUS
BACTERIA
BIOCONVERSION
MICROORGANISMS
ORGANIC COMPOUNDS
SYNTHESIS
140504* - Solar Energy Conversion- Biomass Production & Conversion- (-1989)
550700 - Microbiology