Cell-cell interactions in the osteogenic compartment of bone
The interactions between two different cell populations within the osteogenic compartment have been examined. The proliferation of periosteal fibroblasts (PF) in the presence or absence of osteoblast-like cells (OB), whose proliferative capacity was inhibited by irradiation, was measured. OB stimulated (/sup 3/H)thymidine incorporation in PF and parathyroid hormone (PTH) enhanced the stimulation. In the reverse situation, however, PF inhibited OB. Irradiated OB also stimulated /sup 3/H-thymidine incorporation in OB, and irradiated PF in PF, but both to a lesser extent. Co-culture experiments showed that direct cell-cell contact was a prerequisite for stimulation of PF. Medium mediated contact between physically separated OB and PF did not stimulate, but rather inhibited PF proliferation. These results demonstrate that OB regulate the proliferation of cells in the PF population and can transmit the proliferation stimulating message of PTH to PF. This implies that in vivo the mature osteoblast may play a pivotal role in the (hormonal) regulation of osteoprogenitor cell proliferation and therefore bone formation.
- Research Organization:
- Univ. of Leiden (Netherlands)
- OSTI ID:
- 6745109
- Journal Information:
- Bone; (United States), Vol. 9:2
- Country of Publication:
- United States
- Language:
- English
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59 BASIC BIOLOGICAL SCIENCES
FIBROBLASTS
CELL PROLIFERATION
SKELETON
CELL DIVISION
BIOLOGICAL RADIATION EFFECTS
DNA
PARATHORMONE
THYMIDINE
TRACER TECHNIQUES
TRITIUM COMPOUNDS
ANIMAL CELLS
AZINES
BIOLOGICAL EFFECTS
BODY
CONNECTIVE TISSUE CELLS
HETEROCYCLIC COMPOUNDS
HORMONES
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PEPTIDE HORMONES
PYRIMIDINES
RADIATION EFFECTS
RIBOSIDES
SOMATIC CELLS
560120* - Radiation Effects on Biochemicals
Cells
& Tissue Culture
551001 - Physiological Systems- Tracer Techniques