Monocyte transferrin-iron uptake in hereditary hemochromatosis
Transferrin-iron uptake by peripheral blood monocytes was studied in vitro to test the hypothesis that the relative paucity of mononuclear phagocyte iron loading in hereditary hemochromatosis results from a defect in uptake of iron from transferrin. Monocytes from nine control subjects and 17 patients with hemochromatosis were cultured in the presence of 59Fe-labelled human transferrin. There was no difference in 59Fe uptake between monocytes from control subjects and monocytes from patients with hemochromatosis who had been treated by phlebotomy and who had normal body iron stores. However, 59Fe uptake by monocytes from iron-loaded patients with hemochromatosis was significantly reduced compared with either control subjects or treated hemochromatosis patients. It is likely that this was a secondary effect of iron loading since iron uptake by monocytes from treated hemochromatosis patients was normal. Assuming that monocytes in culture reflect mononuclear phagocyte iron metabolism in vivo, this study suggests that the relative paucity of mononuclear phagocyte iron loading in hemochromatosis is not related to an abnormality in transferrin-iron uptake by these cells.
- Research Organization:
- Department of Medicine, University of Queensland, Royal Brisbane Hospital, Australia
- OSTI ID:
- 6743233
- Journal Information:
- Am. J. Hematol.; (United States), Vol. 16:4
- Country of Publication:
- United States
- Language:
- English
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IRON 59
BIOCHEMICAL REACTION KINETICS
UPTAKE
TRANSFERRIN
CELL CULTURES
HEMIC DISEASES
HEREDITARY DISEASES
MONOCYTES
PHAGOCYTOSIS
TRACER TECHNIQUES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
DAYS LIVING RADIOISOTOPES
DISEASES
EVEN-ODD NUCLEI
GLOBULINS
GLOBULINS-BETA
INTERMEDIATE MASS NUCLEI
IRON ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LEUKOCYTES
MATERIALS
METALLOPROTEINS
NUCLEI
ORGANIC COMPOUNDS
PROTEINS
RADIOISOTOPES
REACTION KINETICS
551001* - Physiological Systems- Tracer Techniques