Evaluation of a cubicle containment system in preventing gaseous and particulate airborne cross-contamination

Journal Article · Thu Dec 01 00:00:00 EST 1983 · Lab. Anim. Sci.; (United States)

OSTI ID:6734132

White, W J; Hughes, H C; Singh, S B; Lang, C M

The effectiveness of a cubicle containment system in preventing gaseous and particulate cross-contamination in animal facilities was evaluated using several techniques. Using a nitrous oxide dilution technique, no airborne cross-contamination was found between cubicles as long as all cubicle doors were kept closed. If the doors to the cubicle in which the gas was released were partially or completely opened, low concentrations of nitrous oxide could be detected in adjacent cubicles. These concentrations increased when the air exchange rates in the cubicle were decreased. Similar results were obtained when particulate transfer was measured using aerosolized Staphlococcus epidermidis and a slit to agar sampling technique. Air flows and point air velocities within the cubicle and the animal room were also studied. A trial of Sendai virus transmission between cubicles revealed no intercubicle transmission after 3 weeks of exposure. Overall, the cubicle containment system appeared to be an effective means of achieving limited biohazard containment, applicable to many research housing needs.

• Research Organization: Pennsylvania State Univ., Hershey
• OSTI ID: 6734132
• Journal Information: Lab. Anim. Sci.; (United States), Vol. 33:6
• Country of Publication: United States
• Language: English

Related Subjects

54 ENVIRONMENTAL SCIENCES
ANIMAL SHELTERS
VENTILATION
AIR FLOW
INDOOR AIR POLLUTION
NITROUS OXIDE
PARTICULATES
AIR POLLUTION
BUILDINGS
CHALCOGENIDES
FLUID FLOW
GAS FLOW
NITROGEN COMPOUNDS
NITROGEN OXIDES
OXIDES
OXYGEN COMPOUNDS
PARTICLES
POLLUTION
SHELTERS
500100* - Environment
Atmospheric- Basic Studies- (-1989)