In vitro and in vivo effect of interleukin-2 on the 2',5'-oligoadenylate synthetase activity of peripheral mononuclear blood cells

Journal Article · Thu Feb 01 00:00:00 EST 1990 · Journal of Interferon Research; (USA)

DOI:https://doi.org/10.1089/jir.1990.10.75· OSTI ID:6727160

Handgretinger, R; Bruchelt, G; Kimmig, A; Lang, P; Daurer, B; Dopfer, R; Treuner, J; Niethammer, D ^[1]

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1. Children's Univ. Hospital, Tuebingen (Germany, F.R.)

The in vitro and in vivo influence of interleukin-2 (IL-2) on 2',5'-oligoadenylate (2-5A) synthetase activity and natural killer (NK) activity of peripheral mononuclear blood cells (PBMCs) was investigated. Incubation of PBMCs in vitro with IL-2 resulted in a considerable secretion of interferon-gamma (IFN-gamma) and in a significant elevation of 2-5A synthetase activity, as well as NK activity. Neutralizing monoclonal anti-IFN-gamma antibodies inhibited the elevation of 2-5A synthetase activity, but not the IL-2-induced augmentation of NK activity. Additionally, 2-5A synthetase and NK activity of PBMCs was measured in a child with neuroblastoma that was treated with recombinant IL-2 by continuous intravenous application. During the treatment, NK activity against the NK-sensitive cell line K 562 and against autologous tumor cells was not augmented. However, a significant increase of 2-5A synthetase activity in PBMCs was observed during IL-2 treatment, whereas there was no detectable serum level of IFN-gamma. We conclude that measuring 2-5A synthetase activity in patients treated with IL-2 may be helpful in monitoring the immunomodulatory effects of IL-2 on immune effector cells.

• OSTI ID: 6727160
• Journal Information: Journal of Interferon Research; (USA), Vol. 10:1; ISSN 0197-8357
• Country of Publication: United States
• Language: English

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