Surface enhanced Raman gene probe and methods thereof
The subject invention disclosed herein is a new gene probe biosensor and methods based on surface enhanced Raman scattering (SERS) label detection. The SER gene probe biosensor comprises a support means, a SER gene probe having at least one oligonucleotide strand labeled with at least one SERS label, and a SERS active substrate disposed on the support means and having at least one of the SER gene probes adsorbed thereon. Biotargets such as bacterial and viral DNA, RNA and PNA are detected using a SER gene probe via hybridization to oligonucleotide strands complementary to the SER gene probe. The support means supporting the SERS active substrate includes a fiberoptic probe, an array of fiberoptic probes for performance of multiple assays and a waveguide microsensor array with charge-coupled devices or photodiode arrays. 18 figs.
- Research Organization:
- Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)
- Sponsoring Organization:
- USDOE, Washington, DC (United States)
- DOE Contract Number:
- AC05-84OR21400
- Assignee:
- Lockheed Martin Energy Systems, Inc., Oak Ridge, TN (United States)
- Patent Number(s):
- US 5,814,516/A/
- Application Number:
- PAN: 8-543,212
- OSTI ID:
- 672677
- Resource Relation:
- Other Information: PBD: 29 Sep 1998
- Country of Publication:
- United States
- Language:
- English
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Surface enhanced Raman gene probe and methods thereof
Surface enhanced Raman gene probe and methods thereof