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Title: Immunoreactive atrial natriuretic factor is increased in ovine model of endotoxemia

Abstract

A bolus of Escherichia coli endotoxin (1.5 {mu}g/kg) was administered to chronically instrumented sheep. Immunoreactive atrial natriuretic factor (IR-ANF) was measured in extracted plasma by radioimmunoassay. There was a thirteenfold increase in IR-ANF 2 h after endotoxin administration, and IR-ANF levels remained significantly elevated during the first 6 h. A marked diuresis and natriuresis occurred between 4 and 6 h. ANF not only affects renal function but is also associated with decreased cardiac output, increased peripheral resistance (in sheep), and decreased capillary absorption (in rats). These renal and hemodynamic changes are also characteristic of the early (first 6 h) response to endotoxin. Therefore ANF should be considered as a potential mediator of renal and hemodynamic changes induced by sepsis. It is difficult to determine if ANF elevation is an epiphenomenon or a causative factor, because no antagonist of ANF is currently available.

Authors:
; ; ; ; ;  [1]
  1. Univ. of Texas Medical Branch, Galveston (USA) Thomas Jefferson Medical College, Philadelphia, PA (USA) Westfaelian Wilhelms Univ., Muenster (West Germany)
Publication Date:
OSTI Identifier:
6724816
Resource Type:
Journal Article
Journal Name:
American Journal of Physiology; (USA)
Additional Journal Information:
Journal Volume: 254:4; Journal ID: ISSN 0002-9513
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; ENDOTOXINS; BIOLOGICAL EFFECTS; PEPTIDES; RADIOIMMUNOASSAY; SEPTICEMIA; IMMUNOLOGY; BLOOD PRESSURE; CREATININE; ESCHERICHIA COLI; EXCRETION; KIDNEYS; POTASSIUM COMPOUNDS; SHEEP; SODIUM COMPOUNDS; URINE; ALKALI METAL COMPOUNDS; ANIMALS; ANTIGENS; AZOLES; BACTERIA; BIOASSAY; BIOLOGICAL MATERIALS; BIOLOGICAL WASTES; BODY; BODY FLUIDS; CLEARANCE; DIAGNOSTIC TECHNIQUES; DOMESTIC ANIMALS; HETEROCYCLIC COMPOUNDS; IMIDAZOLES; IMINES; IMMUNOASSAY; ISOTOPE APPLICATIONS; MAMMALS; MATERIALS; MICROORGANISMS; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; ORGANS; PROTEINS; RADIOASSAY; RADIOIMMUNODETECTION; RADIOIMMUNOLOGY; RUMINANTS; TOXIC MATERIALS; TOXINS; TRACER TECHNIQUES; VERTEBRATES; WASTES; 551001* - Physiological Systems- Tracer Techniques

Citation Formats

Lubbesmeyer, H J, Woodson, L, Traber, L D, Flynn, J T, Herndon, D N, and Traber, D L. Immunoreactive atrial natriuretic factor is increased in ovine model of endotoxemia. United States: N. p., 1988. Web.
Lubbesmeyer, H J, Woodson, L, Traber, L D, Flynn, J T, Herndon, D N, & Traber, D L. Immunoreactive atrial natriuretic factor is increased in ovine model of endotoxemia. United States.
Lubbesmeyer, H J, Woodson, L, Traber, L D, Flynn, J T, Herndon, D N, and Traber, D L. 1988. "Immunoreactive atrial natriuretic factor is increased in ovine model of endotoxemia". United States.
@article{osti_6724816,
title = {Immunoreactive atrial natriuretic factor is increased in ovine model of endotoxemia},
author = {Lubbesmeyer, H J and Woodson, L and Traber, L D and Flynn, J T and Herndon, D N and Traber, D L},
abstractNote = {A bolus of Escherichia coli endotoxin (1.5 {mu}g/kg) was administered to chronically instrumented sheep. Immunoreactive atrial natriuretic factor (IR-ANF) was measured in extracted plasma by radioimmunoassay. There was a thirteenfold increase in IR-ANF 2 h after endotoxin administration, and IR-ANF levels remained significantly elevated during the first 6 h. A marked diuresis and natriuresis occurred between 4 and 6 h. ANF not only affects renal function but is also associated with decreased cardiac output, increased peripheral resistance (in sheep), and decreased capillary absorption (in rats). These renal and hemodynamic changes are also characteristic of the early (first 6 h) response to endotoxin. Therefore ANF should be considered as a potential mediator of renal and hemodynamic changes induced by sepsis. It is difficult to determine if ANF elevation is an epiphenomenon or a causative factor, because no antagonist of ANF is currently available.},
doi = {},
url = {https://www.osti.gov/biblio/6724816}, journal = {American Journal of Physiology; (USA)},
issn = {0002-9513},
number = ,
volume = 254:4,
place = {United States},
year = {Fri Apr 01 00:00:00 EST 1988},
month = {Fri Apr 01 00:00:00 EST 1988}
}