A synthetic peptide corresponding to human FSH. beta. -subunit 33-53 binds to FSH receptor, stimulates basal estradiol biosynthesis, and is a partial antagonist of FSH
- Albany Medical College, NY (USA)
The authors have previously shown that hFSH-{beta} 34-37 (KTCT) and 49-52 (TRDL) inhibit binding of {sup 125}I-hFSH to FSH receptor in calf testis membranes and that hFSH-{beta} 33-53, which encompasses these tetrapeptides, inhibits binding with increased potency. hFSH-{beta} 33-53 rapidly dimerizes under conditions utilized in the receptor binding assay (pH 7.5) so that the binding inhibition reported earlier was due to the hFSH-{beta} 33-53 dimer rather than the monomer. At pH 6.5, conversion to dimer does not occur, and binding inhibition could be unequivocally attributed to the monomer. Radioiodinated and alkylated hFSH-{beta} 33-53 binds to the FSH receptor. The biological activity of hFSH-{beta} 33-53 was assessed by its ability to affect the conversion of androstenedione to estradiol in rat Sertoli cells cultures. This result demonstrates that the free R-SH group at Cys51 is not responsible for the inhibition. FSH-{beta} 33-53 also significantly stimulated basal levels of estradiol synthesis, but not to maximal levels observed with FSH (partial agonist). Neither the carbohydrate content of hFSH-{beta} nor the {alpha} subunit of FSH appears to be essential for signal transduction and expression of the hormone effect of FSH-{beta} 33-53.
- OSTI ID:
- 6719188
- Journal Information:
- Biochemistry; (USA), Vol. 29:5; ISSN 0006-2960
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
ESTRADIOL
BIOSYNTHESIS
FSH
BIOCHEMICAL REACTION KINETICS
PEPTIDES
CROSS-LINKING
RECEPTORS
CALVES
CELL MEMBRANES
INHIBITION
IODINE 125
LIQUID COLUMN CHROMATOGRAPHY
TESTES
ANIMALS
BETA DECAY RADIOISOTOPES
BODY
CATTLE
CELL CONSTITUENTS
CHEMICAL REACTIONS
CHROMATOGRAPHY
DAYS LIVING RADIOISOTOPES
DOMESTIC ANIMALS
ELECTRON CAPTURE RADIOISOTOPES
ESTRANES
ESTROGENS
GONADOTROPINS
GONADS
HORMONES
HYDROXY COMPOUNDS
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOTOPES
KINETICS
MALE GENITALS
MAMMALS
MEMBRANE PROTEINS
MEMBRANES
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANS
PEPTIDE HORMONES
PITUITARY HORMONES
POLYMERIZATION
PROTEINS
RADIOISOTOPES
REACTION KINETICS
RUMINANTS
SEPARATION PROCESSES
STEROID HORMONES
STEROIDS
SYNTHESIS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques