Abnormal segregation of alleles in CEPH pedigree DNAs arising from allele loss in lymphoblastoid DNA

Journal Article · Fri Jan 01 00:00:00 EST 1993 · Genomics; (United States)

DOI:https://doi.org/10.1006/geno.1993.1019· OSTI ID:6717226

Royle, N J; Armour, J A.L.; Crosier, M; Jeffreys, A J ^[1]

---

1. Univ. of Leicester (United Kingdom)

Somatic events that result in the reduction to hemior homozygosity at all loci affected by the event have been identified in lymphoblastoid DNA from mothers of two CEPH families. Using suitably informative probes, the allele deficiencies were detected by the abnormal transmission of alleles from grandparents to grandchildren, with the apparent absence of the alleles from the parent. Undetected somatic deficiencies in family DNAs could result in misscoring of recombination events and consequently introduce errors into linkage analysis. 15 refs., 2 figs.

• OSTI ID: 6717226
• Journal Information: Genomics; (United States), Vol. 15:1; ISSN 0888-7543
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
GENETIC MAPPING
ERRORS
DNA
PROBES
RECOMBINATION
REDUCTION
SEGREGATION
SOMATIC MUTATIONS
TRANSMISSION
CHEMICAL REACTIONS
MAPPING
MUTATIONS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
550400* - Genetics