Formation of DNA adducts in rat lung following chronic inhalation of diesel emissions, carbon black and titanium dioxide particles
The objective of the study was to determine whether the polycyclic aromatic hydrocarbons (PAH), nitro-PAH or other polycyclic organic matter adsorbed to diesel particles induces the formation of DNA adducts in the lung when compared to particles with little or no adsorbed organic matter in conjunction with a chronic inhalation cancer study. Wistar rats were exposed to diesel emissions (7.5 mg/cu m) for 2 months, 6 months and 2 years time points and 2 years to carbon black (11.3 mg/cu m) and TiO2 particles (10.4 mg/cu m) to compare tumorigenic response and DNA adduct formation in the lung. Two versions of the (32)P-postlabeling assay for the detection of DNA adducts were used to identify nirated- or aryl- amine adducts formed relative to other PAH adducts based on the demonstrated sensitivity of these adducts to nuclease P1 treatment. Total adduct levels were determined as well as two specific individual adducts, one major adduct (1) which migrated outside the zone and a nuclease P1 sensitive adduct (2) detected inside the zone. Adduct 1 increased significantly over time in the controls but decreased markedly at the two year time points regardless of particle type. These data suggest that long term contact with these particles may result in a cell of proliferative response, enhanced degradation of I-compounds not related to cell proliferation, and or synthesis of I-compounds, irrespective of the differences in organic content associated with the three particle types.
- Research Organization:
- Environmental Protection Agency, Research Triangle Park, NC (United States). Health Effects Research Lab.
- OSTI ID:
- 6713485
- Report Number(s):
- PB-95-128062/XAB; EPA-600/J-94/420
- Resource Relation:
- Other Information: Pub. in Carcinogenesis, Vol. 15, No. 7, 1291-1299(1994)
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
DIESEL FUELS
GASEOUS WASTES
DNA ADDUCTS
COMPILED DATA
POLYCYCLIC AROMATIC HYDROCARBONS
TOXICITY
ADDUCTS
AROMATICS
DATA
DISTILLATES
ENERGY SOURCES
FOSSIL FUELS
FUELS
GAS OILS
HYDROCARBONS
INFORMATION
LIQUID FUELS
NUMERICAL DATA
ORGANIC COMPOUNDS
PETROLEUM
PETROLEUM DISTILLATES
PETROLEUM FRACTIONS
PETROLEUM PRODUCTS
WASTES
560300* - Chemicals Metabolism & Toxicology