Metabolism of benzo(a)pyrene by isolated perfused testis and testicular homogenate

Journal Article · Mon Dec 01 00:00:00 EST 1980 · Life Sci.; (United States)

DOI:https://doi.org/10.1016/0024-3205(80)90519-6· OSTI ID:6709882

Dixon, R L; Lee, I P

In an effort to improve the extrapolation of laboratory data to man and estimate risk of human reproductive toxicity associated with environmental exposure, the pharmacokinetic parameters of the testicular compartment are being studied. Of particular interest is the variety of enzyme systems capable of activating and detoxicating environmental chemicals and drugs. This report compares the metabolism of benzo(a)pyrene by the isolated perfused testis and testicular homogenates in vitro. The cell free in vitro system metabolized benzo(a)pyrene at a much greater rate than the perfused testis and produced a different spectrum of metabolites. Reliable laboratory prediction of biotransformation by the whole organ or intact animal is an essential aspect of reproductive toxicology.

• Research Organization: National Inst. of Environmental Health Sciences, Research Triangle Park, NC
• OSTI ID: 6709882
• Journal Information: Life Sci.; (United States), Vol. 27:25/26
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
59 BASIC BIOLOGICAL SCIENCES
BENZOPYRENE
METABOLISM
TOXICITY
TESTES
SENSITIVITY
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL PATHWAYS
CELL CULTURES
COMPARATIVE EVALUATIONS
DYNAMIC FUNCTION STUDIES
EXTRAPOLATION
HOMOGENATES
IN VITRO
METABOLITES
PERFUSED TISSUES
REPRODUCTION
ANIMAL TISSUES
AROMATICS
BODY
CONDENSED AROMATICS
GONADS
HYDROCARBONS
KINETICS
MALE GENITALS
NUMERICAL SOLUTION
ORGANIC COMPOUNDS
ORGANS
REACTION KINETICS
TISSUES
560301* - Chemicals Metabolism & Toxicology- Cells- (-1987)
551000 - Physiological Systems