Genotoxic effects of sunlight-activated waste water in cultured mammalian cells
Cultured Chinese hamster ovary cells were incubated with dilutions of an oil shale retort process water and exposed to nautral sunlight. An enhancement of sevenfold to ninefold was seen in photoinduced cytotoxicity (by a colony-forming assay) and mutagenicity (at the hypoxanthine phosphoribosyltransferase (HPRT) locus) for cells pretreated with the process water compared to effects seen in cells exposed to sunlight only. Significant photoinduced cytotoxicity was also observed in cultured human skin fibroblasts when exposed to the process water before being exposed to near UV (NUV) radiation. The mutation frequencies (determined for the HPRT locus) induced by the process water and NUV radiation were as great as those frequencies seen for far UV light alone. Increases in genotoxicity were observed in excision repair-deficient xeroderma pigmentosum skin fibroblasts when compared to the responses seen in normal cells. Risks to health resulting from the phototransformation of these oil shale retort process waste waters are unassessed at this time.
- Research Organization:
- Los Alamos National Laboratory, NM
- OSTI ID:
- 6707380
- Journal Information:
- JNCI, J. Natl. Cancer Inst.; (United States), Vol. 69:1
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
04 OIL SHALES AND TAR SANDS
CELL CULTURES
SENSITIVITY
OIL SHALES
TOXICITY
FIBROBLASTS
MUTAGENESIS
MUTATION FREQUENCY
NEAR ULTRAVIOLET RADIATION
OVARIES
PHOSPHOTRANSFERASES
PHOTOSENSITIVITY
SKIN
VISIBLE RADIATION
WASTE WATER
ANIMAL CELLS
BITUMINOUS MATERIALS
BODY
CARBONACEOUS MATERIALS
CONNECTIVE TISSUE CELLS
ELECTROMAGNETIC RADIATION
ENERGY SOURCES
ENZYMES
FEMALE GENITALS
FOSSIL FUELS
FUELS
GONADS
HYDROGEN COMPOUNDS
LIQUID WASTES
MATERIALS
ORGANS
OXYGEN COMPOUNDS
PHOSPHORUS-GROUP TRANSFERASES
RADIATIONS
SOMATIC CELLS
TRANSFERASES
ULTRAVIOLET RADIATION
WASTES
WATER
560301* - Chemicals Metabolism & Toxicology- Cells- (-1987)
040700 - Oil Shales & Tar Sands- Health & Safety