Using miniature osmotic infusion pumps to maintain tritiated thymidine exposure to cells in culture

Journal Article · Tue Jun 01 00:00:00 EDT 1982 · J. Histochem. Cytochem.; (United States)

DOI:https://doi.org/10.1177/30.6.7047639· OSTI ID:6706212

Neely, J E; Hake, D A

To provide a constant level of tracer doses of tritiated thymidine to cultured cells during continuous infusion, miniature osmotic infusion pumps were used to provide replacement thymidine. By determining the loss of isotope from the media during nonreplacement, the rate of constant infusion replacement to maintain thymidine levels was calculated. The replacement rates were similar for the three cell lines examined and allowed a standard osmotic pump infusion.

• Research Organization: Division of Hematology, Children's Hospital Research Foundation, Cincinnati, Ohio
• OSTI ID: 6706212
• Journal Information: J. Histochem. Cytochem.; (United States), Vol. 30:6
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
THYMIDINE
INFUSION
TRITIUM COMPOUNDS
LABELLING
CELL CULTURES
CULTURE MEDIA
ISOTOPE APPLICATIONS
OSMOSIS
PERFORMANCE TESTING
PUMPS
RADIATION DOSES
AZINES
DIFFUSION
DOSES
HETEROCYCLIC COMPOUNDS
INTAKE
LABELLED COMPOUNDS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PYRIMIDINES
RIBOSIDES
TESTING
550201* - Biochemistry- Tracer Techniques